High genetic variability in an RNA plant virus, banana mild mosaic virus

Teycheney Pierre-Yves, Laboureau Nathalie, Iskra Caruana Marie-Line, Candresse Thierry. 2005. High genetic variability in an RNA plant virus, banana mild mosaic virus. In : Microbes in a changing world = XIIIth International Congress of Virology, San Fransisco (USA), July 23-28, 2005. s.l. : s.n., 2 p. International Congress of Virology. 13, San Francisco, États-Unis, 23 Juillet 2005/28 Juillet 2005.

Résumé : Background: Banana Mild Mosaic virus (BanMMV) is an unassigned member of the Flexiviridae family infecting banana and plantain (Musa spp). Vertical transmission by vegetative propagation is currently the only known means of propagation of this virus, since no biological vector has been identified nor has experimental mechanical inoculation on susceptible hosts been successful so far. Methods: Nucleotide sequences were obtained from BanMMV isolates originating from various locations worldwide. Two distinct regions of the viral genome were targeted: ORF 1, which encodes the viral RNA-dependent RNA polymerase (RdRp), and a region encompassing the 3' end of ORF5, which encodes the coat protein (CP), and the 3' non-coding region (3' NCR). Both regions play a key role in viral replication and are therefore supposedly very conserved. Results: BanMMV RdRp sequences displayed an extremely high level of molecular diversity when compared to similar sequences obtained from other plant RNA viruses. This situation resulted essentially from a very high ratio of synonymous mutations. A similar analysis performed for a limited selection of banana accessions on the CP/3'NCR region confirmed the high level of diversity observed in the RdRp dataset, characterized by a very high level of synonymous mutations. Analysis of intra-host diversity indicated the existence of two distinct situations with some plants harbouring only closely related sequence variants while others contained up to three widely divergent isolates. Analyses indicated an absence of structuration of the virus genetic diversity either by the geographical origin of the infected Musa accessions or by their genotype. This situation may be, in part, explained by the exchange of banana germplasm between different parts of the world and also by plant-to-plant transmission of the virus, the evidence for which is, for the first time, provided by this study.

Mots-clés Agrovoc : Musa, virus des végétaux, variation génétique, ressource génétique, arn, transmission des maladies, multiplication des plantes, vecteur de maladie

Mots-clés complémentaires : Virus de la mosaïque, Flexiviridae, Variabilité génétique

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

Auteurs et affiliations

• Teycheney Pierre-Yves, CIRAD-FLHOR-UPR Multiplication végétative (GLP) ORCID: 0000-0002-9754-0745
• Laboureau Nathalie, CIRAD-AMIS-UMR BGPI (FRA) ORCID: 0000-0002-0105-3919
• Iskra Caruana Marie-Line, CIRAD-AMIS-UMR BGPI (FRA) ORCID: 0000-0003-4486-2449
• Candresse Thierry, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/527712/)

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