Stable resistance against the leaf miner Leucoptera coffeella expressed by genetically transformed Coffea canephora in a pluriannual field experiment in French Guiana

Perthuis Bernard, Pradon Jean-Louis, Montagnon Christophe, Dufour Magali, Leroy Thierry. 2005. Stable resistance against the leaf miner Leucoptera coffeella expressed by genetically transformed Coffea canephora in a pluriannual field experiment in French Guiana. Euphytica, 144 : 321-329.

Résumé : A pluriannual field trial of transgenic clones of Coffea canephora (the Robusta coffee tree) transformed for resistance to the lepidopteran coffee leaf miner Leucoptera coffeella was installed in French Guiana. Fifty-eight transformed clones produced by transformation of the C. canephora clone 126 were planted. They were harbouring the pEF1 [alpha] constitutive promoter of Arabidopsis thaliana controlling either the Bacillus thuringiensis native gene for the cry 1 Ac insecticidal protein (eight clones) or a synthetic crylAc gene (53 clones). The vectors for the transformation were a strain of the bacterium Agrobacterium tumefaciens and one of Agrobacterium rhizogenes. The transformed clones were generally independent, presenting different integration patterns of the genetic construct. Four randomly distributed groups of five plants per transformed clone were planted along with 60 untransformed control trees. Over a 4-year period after plantation six releases of L. coffeella were performed. Mines on the leaves are the marks of larvae development and were counted on plants. A majority of the independent transformed clones harbouring the synthetic gene and transformed by the strain of A. tumefaciens displayed constantly much less mines than the control, therefore expressing a stable resistance. The need for complementary research is presented.

Mots-clés Agrovoc : Leucoptera, Coffea canephora, résistance aux organismes nuisibles, transfert de gène, organisme génétiquement modifié, expression des gènes, stabilité, expérimentation au champ

Mots-clés géographiques Agrovoc : Guinée

Classification Agris : F30 - Génétique et amélioration des plantes
H10 - Ravageurs des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Perthuis Bernard, CIRAD-AMIS-UMR PIA (GUF)
• Pradon Jean-Louis, CIRAD-CP-US Services en Guyane (GUF)
• Montagnon Christophe, CIRAD-CP-DIR (FRA)
• Dufour Magali, CIRAD-AMIS-UMR PIA (FRA)
• Leroy Thierry, CIRAD-AMIS-UMR PIA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/529094/)

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