Efficiency and limits of the serial analysis of gene expression (SAGE) method : Discussions based on first results in bovine trypanotolerance

Maillard Jean-Charles, Berthier David, Thevenon Sophie, Piquemal David, Chantal Isabelle, Marti Jacques. 2005. Efficiency and limits of the serial analysis of gene expression (SAGE) method : Discussions based on first results in bovine trypanotolerance. Veterinary Immunology and Immunopathology, 108 (1-2) : 59-69. International Veterinary Immunology Symposium (IVIS). 7, Québec, Canada, 25 Juillet 2004/30 Juillet 2004.

https://doi.org/10.1016/j.vetimm.2005.08.005

Article de revue ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_530085.pdf
Télécharger (234kB)

Résumé : Post genomic biotechnologies, such as transcriptome analysis, are now efficient enough to characterize the full complement of genes involved in the expression of specific biological functions. One of them is the Serial Analysis of Gene Expression (SAGE) technique. SAGE involves the construction of transcript libraries for a quantitative analysis of the entire set of genes expressed or inactivated at particular stages of cellular activation. Bioinformatic comparisons in hosts and pathogens genomic databases allow the identification of several up- and down-regulated genes, ESTs and unknown transcripts directly involved in the host¿pathogen immunological interaction mechanisms. Based on the first results obtained during an experimental Trypanosoma congolense infection in trypanotolerant cattle, the efficiency and limits of such a technique, from the data acquisition level to the data analysis level, is discussed in this analysis.

Mots-clés Agrovoc : méthode, expression des gènes, Trypanosoma congolense, analyse biologique, réponse immunitaire, race (animal)

Mots-clés complémentaires : Trypanotolérance, Bovin N'Dama

Classification Agris : L50 - Physiologie et biochimie animales

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations