Organ-specific expression of IGF-I during early development of bony fish as revealed in the tilapia, Oreochromis niloticus, by in situ hybridization and immunohistochemistry : indication for the particular importance of local IGF-I

Berishvili Giorgi, Shved Natallia, Eppler Elisabeth, Clota Frédéric, Baroiller Jean-François, Reinecke Manfred. 2006. Organ-specific expression of IGF-I during early development of bony fish as revealed in the tilapia, Oreochromis niloticus, by in situ hybridization and immunohistochemistry : indication for the particular importance of local IGF-I. Cell and Tissue Research, 325 : 287-301.

Résumé : The cellular sites of insulin-like growth factor I (IGF-I) synthesis in the early developing tilapia (0-140 days post fertilization, DPF) were investigated. IGF-I mRNA and peptide appeared in liver as early as 4 DPF and in gastro-intestinal epithelial cells between 5-9 DPF. In exocrine pancreas, the expression of IGF-I started at 4 DPF and continued until 90 DPF. IGF-I production was detected in islets at 6 DPF in non-insulin cells and occurred throughout life. In renal tubules and ducts, IGF-I production started at 8 DPF. IGF-I production in chondrocytes had its onset at 4 DPF, was more pronounced in growing regions and was also found in adults. IGF-I mRNA and peptide appeared in the cytoplasm of skeletal muscle cells at 4 DPF. In gill chloride cells, IGF-I production started at 6 DPF. At 13 DPF, IGF-I was detected in cardiac myocytes. IGF-I-producing epidermal cells appeared at 5 DPF. In brain and ganglia, IGF-I was expressed in virtually all neurones from 6 to 29 DPF, their number decreasing with age. Neurosecretory IGF-I-immuno reactive axons were first seen in the neurohypophysis around 17 DPF. Endocrine cells of the adenohypophysis exhibited IGF-I mRNA at 28 DPF and IGF-I immuno reactivity at 40 DPF. Thus, IGF-I appeared early (4-5 DPF), first in liver, the main source of endocrine IGF-I, and then in organs involved in growth or metabolism. The expression of IGF-I was more pronounced during development than in juvenile and adult life. Local IGF-I therefore seems to have a high functional impact in early growth, metabolism and organogenesis.

Mots-clés Agrovoc : Oreochromis niloticus, Tilapia, hybridation, fertilisation, cellule

Mots-clés complémentaires : Immunocytochimie

Classification Agris : M12 - Production de l'aquaculture

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Berishvili Giorgi, UZH (CHE)
• Shved Natallia, UZH (CHE)
• Eppler Elisabeth, UZH (CHE)
• Clota Frédéric, CIRAD-EMVT-UPR Aquaculture (FRA)
• Baroiller Jean-François, CIRAD-EMVT-UPR Aquaculture (FRA) ORCID: 0000-0002-0992-1725
• Reinecke Manfred, UZH (CHE)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/533223/)

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