In situ expression of two storage protein genes in relation to histo-differentiation at mid-embryogenesis in Medicago truncatula and Pisum sativum seeds

Abirached-Darmency M., Abdel-Gawwad M.R., Conejero Geneviève, Verdeil Jean-Luc, Thompson R.. 2005. In situ expression of two storage protein genes in relation to histo-differentiation at mid-embryogenesis in Medicago truncatula and Pisum sativum seeds. Journal of Experimental Botany, 56 (418) : 2019-2028.

Résumé : The seed consists of several layers of specialized cell-types that divide and differentiate following a highly regulated programme in time and space. A cytological approach was undertaken in order to study the histo-differentiation at mid-embryogenesis in Medicago truncatula as a model legume, and in Pisum sativum using serial sections of embedded immature seed. Little published information is available about seed development in Medicago species. The observations from this study revealed a number of distinctive features of Medicago seed development and differentiation. Trans-fer cells, involved in nutrient transfer to the embryo, were clearly identified in the thin-walled parenchyma of the innermost integument. Histological Schiff-naphthol enabled carbohydrate accumulation to be followed in the different seed compartments, and revealed the storage protein bodies. Non-radioactive mRNA in situ hybridization, was carried out using mRNA probes from two highly expressed genes encoding the major vicilin and legumin A storage protein types. The timing of mRNA expression was related to that of the corresponding proteins already identified.

Mots-clés Agrovoc : Medicago truncatula, Pisum sativum, semence, différenciation cellulaire, développement embryonnaire, hybridation, arn messager, globuline, maturation, marqueur génétique, expression des gènes, protéine de réserve

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Abirached-Darmency M., INRA (FRA)
• Abdel-Gawwad M.R., INRA (FRA)
• Conejero Geneviève
• Verdeil Jean-Luc, CIRAD-CP-UMR BEPC (FRA)
• Thompson R., INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/533467/)

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