Sperm survival during short-term storage and after cryopreservation of semen from striped trumpeter (Latris lineata)

Ritar A.J., Campet Marc. 2000. Sperm survival during short-term storage and after cryopreservation of semen from striped trumpeter (Latris lineata). Theriogenology, 54 : 467-480.

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Methods of short-term storage and cryopreservation were examined for semen from striped trumpeter (Laths lineata). For fresh semen at 18°C, the percentage of motile sperm declined rapidly from over 80% immediately after activation with sea water to less than 2% within 9 min after activation The motility after activation of undiluted fresh sperm stored at 5°C was maintained for two days and then declined markedly so that by the eighth day, sperm were mostly immotile after activation The post-thawing motility was higher for sperm frozen with a non-activating diluent containing 2 84 M DMSO in saline (117 mM NaCl) than in an activating glycerol (2 M) medium in dilute sea water (300 mOsm). Post-thawing motility was higher for a dilution rate of 1:5 (semen:diluent) than 1 2 or 1.11 but was similar when frozen semen was thawed at 10°, 20° or 30°C For semen stored at a range of volumes as pellets frozen on dry ice (0.2 to 2 0 mL) or straws frozen in liquid nitrogen vapor (0 25 to 0 5 mL) and thawed in a waterbath at 20°C, the post-thawing motilities were similar even though the patterns of cooling and thawing differed markedly between methods of freezing and sizes of pellets and straws. ©2000 by Elsevier Science Inc

Mots-clés Agrovoc : Latris lineata, reproduction, insémination artificielle, cryoprotecteur

Classification Agris : M12 - Production de l'aquaculture
L53 - Physiologie animale - Reproduction

Auteurs et affiliations

• Ritar A.J., Tasmanian Aquaculture and Fisheries Institute (AUS)
• Campet Marc, CIRAD-EMVT-PPA (VNM)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/535470/)

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