Expression of the rice vdac isoform2 : histochemical localization and expression level

Al Bitar Fawaz, Roosens Nancy, Van Boxtel Jos, Dewaele Eric, Jacobs Michel, Homblé Fabrice. 2002. Expression of the rice vdac isoform2 : histochemical localization and expression level. Biochimica and Biophysica Acta, N. Gene structure and Expression, 1579 : 133-141.

Résumé : The voltage-dependent anion-selective channel (VDAC) is a mitochondrial outer membrane ion channel. The putative promoter of the rice vdac isoform2 (osvdac2) was isolated by screening a rice genomic library. Computer-based analyses predicted a TATA box, a putative transcription start and several transcription factor-binding sites including pollen specific elements. The promoter region was fused to the gus reporter gene and introduced into rice by Agrobacterium-mediated transformation. Histochemical and cell-type localizations indicated an overall expression of this promoter with a strong expression in actively growing lateral roots and in the pollen grains. Quantitative experiments showed that the osvdac2 promoter has a strong specific activity in both root and shoot. Thus, the osvdac2 promoter could be a good alternative to viral promoters (e.g. CaMV 35S) to overexpress genes in transgenic Poaceae. © 2002 Elsevier Science B.V. All rights reserved.

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement

Auteurs et affiliations

• Al Bitar Fawaz, ULB (BEL)
• Roosens Nancy, Vrije Universiteit Brussel (BEL)
• Van Boxtel Jos, CIRAD-AMIS-BIOTROP (FRA)
• Dewaele Eric, Vrije Universiteit Brussel (BEL)
• Jacobs Michel, Vrije Universiteit Brussel (BEL)
• Homblé Fabrice, ULB (BEL)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/536095/)

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