Mapping and structural analysis of B-cell epitopes on the morbillivirus nucleoprotein amino terminus

Bodjo Sanne Charles, Kwiatek Olivier, Diallo Adama, Albina Emmanuel, Libeau Geneviève. 2007. Mapping and structural analysis of B-cell epitopes on the morbillivirus nucleoprotein amino terminus. Journal of General Virology, 88 : 1231-1242.

Résumé : By analysing the antigenic structure of the morbillivirus nucleoprotein (N) using a competitive-binding assay of monoclonal antibodies (mAbs), six different antigenic sites were identified previously. By using Pepscan methodology complemented by analysis of truncated N proteins, a better characterization of five of these antigenic sites was provided: I, II, III, IV and VI. mAbs specific to Rinderpest virus, defining antigenic sites II, III and IV, and those common to four morbilliviruses, delineating sites I and VI, were analysed in the present study. It was found that all but one mapped to the same region, between aa 120 and 149 of N. However, the mAb 3-1 epitope was located in the carboxy-terminal region (aa 421-525). This result may indicate the high immunogenicity of the amino-terminal variable region, at least in the mouse. It was surprising that the epitope of mAb 33-4, antigenic site VI, which recognized all morbilliviruses so far tested, was located in one of the two non-conserved regions between morbillivirus N proteins. It is shown that the conserved amino acid motif 126EAD128----131F-------148EN149 is critical for epitope constitution and recognition.

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Bodjo Sanne Charles, IAEA (AUT)
• Kwiatek Olivier, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
• Diallo Adama, CIRAD-EMVT-UPR Contrôle des maladies (AUT)
• Albina Emmanuel, CIRAD-EMVT-UPR Contrôle des maladies (FRA)
• Libeau Geneviève, CIRAD-EMVT-UPR Contrôle des maladies (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/537516/)

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