Cross-amplification tests of ungulate primers in roe deer (Capreolus capreolus) to develop a multiplex panel of 12 microsatellite loci

Galan M., Cosson Jean François, Aulagnier Stéphane, Maillard Jean-Charles, Thevenon Sophie, Hewison A.J.Marc. 2003. Cross-amplification tests of ungulate primers in roe deer (Capreolus capreolus) to develop a multiplex panel of 12 microsatellite loci. Molecular Ecology Notes, 3 : 142-146.

https://doi.org/10.1046/j.1471-8286.2003.00384.x

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Résumé : Cross-amplification permits transposition of microsatellite markers to closely related species. Multiplexing reduces time and cost further, exploiting simultaneous amplification of several primers. In cross-amplification tests of 55 ungulate primers in roe deer, 39 gave specific amplification products and 20 were polymorphic. Twelve primers were retained to form a multiplex kit. In 30 roe deer, average allelic diversity was 5.67 (range: 2-15), expected heterozygosity was 0.664 and mean polymorphic information content (PIC) value was 0.605. Probability of identity was P ID = 5 X 10-11 and probability of exclusion for parentage studies was P E1 = 0.98856 and P E2 = 0.99952.

Mots-clés Agrovoc : Bovidae, Cervidae, PCR, microsatellite, chevreuil

Classification Agris : L50 - Physiologie et biochimie animales

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