Targeted transcriptome profiling during seed development in Coffea arabica cv. Laurina

Joët Thierry, Salmona Jordi, Descroix Frédéric, Dussert Stéphane, Bertrand Benoît, Noirot Michel. 2007. Targeted transcriptome profiling during seed development in Coffea arabica cv. Laurina. In : 21st International Conference on Coffee Science, Montpellier (France), 11th - 15th September 2006 = 21ème Colloque scientifique international sur le café ; 21. Internationales Wissenshaftliches Kolloquium über Kaffee ; 21e Coloquio Cientifico Internacional sobre el Café. ASIC. Montpellier : ASIC, 687-694. ISBN 2-900212-20-0 Colloque Scientifique International sur le Café. 21, Montpellier, France, 11 Septembre 2006/15 Septembre 2006.

Résumé : Due to its economic importance, Coffea is receiving increasing attention with respect to genomic research. As a first approach on coffee seed development, we carried out a preliminary targeted transcriptomic study using carefully selected cDNAs. On the basis of EST libraries generated from mRNAs of C. canephora and C. arabica fruits and leaves we focused on cDNAs encoding enzymes potentially involved in seed development and/or in biosynthesis of precursors playing a role in beverage quality. We selected 266 candidate genes that were PCR-amplified and spotted onto a nylon membrane. So obtained cDNA arrays were used to screen the transcriptional status of the endosperm of C. arabica cv. Laurina grown in La Réunion Island, at seven different development stages (sampled between 60 and 250 days after flowering). Clustering analysis was performed using gene expression data and was used to highlight observed phenological changes. Analysis of variance sorted seventy five cDNAs differentially expressed during seed development. We classified these genes into five distinct groups of expression pattern through multivariate analysis.

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement

Auteurs et affiliations

• Joët Thierry, CIRAD-CP-UMR DGPC (FRA)
• Salmona Jordi, CIRAD-CP-UMR DGPC (FRA)
• Descroix Frédéric, CIRAD-CP-UPR Qualité produits pérennes (REU)
• Dussert Stéphane, IRD (FRA)
• Bertrand Benoît, CIRAD-CP-UMR DGPC (FRA) ORCID: 0000-0003-1969-3479
• Noirot Michel, CIRAD-CP-UMR DGPC (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/540072/)

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