Control of ruminant morbillivirus replication by small interfering RNA

Servan de Almeida Renata, Keita Djénéba, Libeau Geneviève, Albina Emmanuel. 2007. Control of ruminant morbillivirus replication by small interfering RNA. Journal of General Virology, 88 : pp. 2307-2311.

Journal article ; Article de revue à facteur d'impact
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Abstract : Peste-des-petits-ruminants virus (PPRV) and rinderpest virus (RPV) are two morbilliviruses of economic relevance in African and Asian countries. Although efficient vaccines are available for both diseases, they cannot protect the animals before 14 days post-vaccination. In emergencies, it would be desirable to have efficient therapeutics for virus control. Here, two regions are described in the nucleocapsid genes of PPRV and RPV that can be targeted efficiently by synthetic short interfering RNAs (siRNAs), resulting in a >80% reduction in virus replication. The effects of siRNAs on the production of viral RNA by real-time quantitative PCR, of viral proteins by flow cytometry and of virus particles by appreciation of the cytopathic effect and virus titration were monitored. The findings of this work highlight the potential for siRNA molecules to be developed as therapeutic agents for the treatment of PPRV and RPV infections. © 2007 by the Society for General Microbiology.

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Servan de Almeida Renata, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
  • Keita Djénéba
  • Libeau Geneviève, CIRAD-BIOS-UPR Contrôle des maladies (FRA)
  • Albina Emmanuel, CIRAD-BIOS-UPR Contrôle des maladies (FRA)

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