Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater

Marcelino Isabel, Vachiery Nathalie, Amaral Ana Isabel, Roldao Antonio, Lefrançois Thierry, Carrondo Manuel J.T., Alves Paula M., Martinez Dominique. 2007. Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater. Vaccine, 25 : 4903-4913.

Résumé : This work evaluates the effect of purification process and storage conditions (buffer formulation and temperature) on the efficacy of Ehrlichia ruminantium (ER) elementary bodies to be used as an inactivated vaccine candidate against heartwater. In vitro assays revealed that, to avoid major losses in ER integrity and corresponding antigenic properties, a buffer with pH between 5.6 and 8 and an osmolality above 100 mOsmol/kg H2O is recommended. Amongst the tested formulations, both PBS and NaCl have shown to stabilize ER antigens at -20°C. To assess the protective properties of the different vaccine formulations, in vivo experiments were performed using a goat model. The results obtained showed that the preparation of ER antigens using a novel membrane-based purification strategy and a simple vaccine formulation (NaCl, -20°C) induced equivalent protection to the conventional vaccine based on ER antigens prepared by a multistep centrifugation methodology and stored at -20°C in PBS buffer. © 2007 Elsevier Ltd. All rights reserved.

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Marcelino Isabel, IBET (PRT)
• Vachiery Nathalie, CIRAD-BIOS-UPR Contrôle des maladies (GLP)
• Amaral Ana Isabel, IBET (PRT)
• Roldao Antonio, IBET (PRT)
• Lefrançois Thierry, CIRAD-BIOS-UPR Contrôle des maladies (GLP) ORCID: 0000-0001-8793-5228
• Carrondo Manuel J.T., IBET (PRT)
• Alves Paula M., IBET (PRT)
• Martinez Dominique, CIRAD-BIOS-UMR CMAEE (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/540385/)

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