Froelicher Yann, Bassene Jean Baptiste, Jedidi Néji Emna, Dambier Dominique, Morillon Raphaël, Bernardini Geneviève, Costantino Gilles, Ollitrault Patrick. 2007. Induced parthenogenesis in mandarin for haploid production : Induction procedures and genetic analysis of plantlets. Plant Cell Reports, 26 (7) : 937-944.
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Résumé : This study focused on haploid induction in mandarin through in situ gynogenesis by pollination with irradiated pollen of 'Meyer' lemon. Pollination was carried out for three genotypes of mandarin with four levels of gamma-ray-irradiated pollen (150, 300, 600, and 900 Gy). The resulting seeds were characterised by a small size. Embryos were rescued in vitro and the ploidy level of the plantlets was determined by flow cytometry analysis. Haploid, diploid, triploid plantlets were obtained. The haploid parthenogenetic origin was confirmed using microsatellite marker analysis and chromosome count. Diploid and triploid plants were the result of crosses between mandarin and lemon. The induction of gynogenetic haploids of 'Fortune' (Citrus clementina Hort ex Tan. × Citrus tangerina Hort ex Tan.) and 'Ellendale' (Citrus reticulata Blanco × Citrus sinensis L. Osb) is reported here for the first time.
Mots-clés Agrovoc : Citrus, parthénogénèse, amélioration des plantes, gynogénèse, haploïdie, marqueur génétique, microsatellite
Mots-clés complémentaires : Induction, Plante haploïde
Classification Agris : F63 - Physiologie végétale - Reproduction
F30 - Génétique et amélioration des plantes
Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique
Auteurs et affiliations
- Froelicher Yann, CIRAD-BIOS-UPR Multiplication végétative (FRA)
- Bassene Jean Baptiste, CIRAD-BIOS-UPR Multiplication végétative (ESP)
- Jedidi Néji Emna, INRAT (TUN)
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Dambier Dominique, CIRAD-BIOS-UPR Multiplication végétative (FRA)
ORCID: 0000-0002-9117-8853
- Morillon Raphaël, CIRAD-BIOS-UPR Multiplication végétative (ESP)
- Bernardini Geneviève, INRA (FRA)
- Costantino Gilles, INRA (FRA)
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Ollitrault Patrick, CIRAD-BIOS-UPR Multiplication végétative (ESP) ORCID: 0000-0002-9456-5517
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/540749/)
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