Unfertilized ovary : a novel explant for coconut (Cocos nucifera L.) somatic embryogenesis

Perera Prasanthi I.P., Hocher Valérie, Verdeil Jean-Luc, Doulbeau Sylvie, Yakandawala Deepthi M.D., Weerakoon L. Kaushalya. 2007. Unfertilized ovary : a novel explant for coconut (Cocos nucifera L.) somatic embryogenesis. Plant Cell Reports, 26 : 21-28.

Résumé : Unfertilized ovaries isolated from immature female flowers of coconut (Cocos nucifera L.) were tested as a source of explants for callogenesis and somatic embryogenesis. The correct developmental stage of ovary explants and suitable in vitro culture conditions for consistent callus production were identified. The concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) and activated charcoal was found to be critical for callogenesis. When cultured in a medium containing 100 [mu]M 2,4-D and 0.1% activated charcoal, ovary explants gave rise to 41% callusing. Embryogenic calli were sub-cultured into somatic embryogenesis induction medium containing 5 [mu]M abscisic acid, followed by plant regeneration medium (with 5 [mu]M 6-benzylaminopurine). Many of the somatic embryos formed were complete with shoot and root poles and upon germination they gave rise to normal shoots. However, some abnormal developments were also observed. Flow cytometric analysis revealed that all the calli tested were diploid. Through histological studies, it was possible to study the sequence of the events that take place during somatic embryogenesis including orientation, polarization and elongation of the embryos.

Mots-clés Agrovoc : Cocos nucifera, Embryogénèse somatique, culture d'organe, gynécée, tissu végétal, physiologie végétale, culture in vitro, numération cellulaire, régénération in vitro, développement biologique, callogénèse, histologie

Classification Agris : F02 - Multiplication végétative des plantes
F30 - Génétique et amélioration des plantes
F63 - Physiologie végétale - Reproduction

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Perera Prasanthi I.P., CRI [Sri Lanka] (LKA)
• Hocher Valérie, IRD (FRA)
• Verdeil Jean-Luc, CIRAD-CP-UMR BEPC (FRA)
• Doulbeau Sylvie, IRD (FRA)
• Yakandawala Deepthi M.D., University of Peradeniya (LKA)
• Weerakoon L. Kaushalya, CRI [Sri Lanka] (LKA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/540880/)

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