Genetic and temperature sex determination in the turbot Scophthalmus maximus

Lebègue Elisabeth, Jeu Sylvie, Guennoc Michel, Guiguen Yann, Baroiller Jean-François, Haffray Pierrick. 2007. Genetic and temperature sex determination in the turbot Scophthalmus maximus. Aquaculture, 272suppl.1, Résumé, S264 International Symposium on Genetics in Aquaculture. 9, Montpellier, France, 26 Juin 2006/30 Juin 2006.

Résumé : Genetic (GSD) and temperature sex determination (TSD) have been investigated in the turbot, Scophthalmus maximus using sex inversed males (androgen treated males=ATM) and females (oestradiol treated female-s= OTF). ATM and OTF have been produced through feeding treatments using 17[alpha]-methyltestosterone and 17[bêta]-oestradiol at a dosage of 3 or 5 mg/kg food starting from 35 days post fertilization (dpf) during periods of 500 or 700° days. 16 ATM and 6 OTF were mated with normal females or males. The 22 resulting progenies and 11 control families were reared at 17°C. For TSD evaluation, parts of 8 of these 33 families (2 from ATM and 6 from controls) have been reared at 15°C, 17°C or 24°C from 35 dpf to 100 dpf. Then all progenies (GSD and TSD) have been reared at 17°C until 11 months old (100 g body weight) for the sex ratio assessment. For 27 progenies reared at 17°C, sex ratios perfectly fit in with the hypothesis of a male homogamety ZZ/ZW system (X-square test; P<0.01). However, 2 ATM, 2 OTF and 2 control progenies presented skewed sex ratios with an excess of males (65-80%). Finally, 24 C treatments significantly affected 3 out of the 8 progenies evaluated for TSD in decreasing the rate of males. It is concluded that in turbot sex is mainly determined by a pair of sex chromosome with a female heterogamety (ZZ/ZW) but sex ratios may also be modulated by parental factors, high temperature and possible temperature/genotype interactions. This project was partly supported by France Turbot and the EU in the PL 97-3796 FAIR Project.

Classification Agris : M12 - Production de l'aquaculture

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Lebègue Elisabeth
• Jeu Sylvie
• Guennoc Michel
• Guiguen Yann, INRA (FRA)
• Baroiller Jean-François, CIRAD-PERSYST-UPR Aquaculture (FRA) ORCID: 0000-0002-0992-1725
• Haffray Pierrick, SYSAAF (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/541994/)

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