Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite vietnamese rice (Oryza sativa L.) cultivars

Ho N.H., Baisakh N., Oliva N., Datta K., Frutos Roger, Datta S.K.. 2006. Translational fusion hybrid Bt genes confer resistance against yellow stem borer in transgenic elite vietnamese rice (Oryza sativa L.) cultivars. Crop Science, 46 : 781-789.

Résumé : We have shown the efficacies of translationally fused gene (cry1Ab-1B) under the control of maize ubiquitin promoter and hybrid Bt gene (cry1A/cry1Ac) driven by rice Actin-1 promoter against the yellow stem borer (Scirpophaga incertulas Walker, YSB) insect in rice (Oryza sativa L.). The integration, expression, and functionality of the transgenes were confirmed by Southern, western, and insect bioassay. The bioassay results correlated with the molecular data. The amount of Bt protein (within the range of 0.8-1.3% of the total soluble protein) in the transgenics was sufficient to cause 100% mortality of the neonate larvae of YSB within 1 wk of infestation. The transgenic plants expressing the foreign Bt protein were normal in phenotype with as good seed setting as the nontransgenic control plants.

Mots-clés Agrovoc : Oryza sativa, Scirpophaga incertulas

Mots-clés géographiques Agrovoc : Viet Nam

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Ho N.H., Institute of Tropical Biology (VNM)
• Baisakh N., Louisiana State University Agricultural Center (USA)
• Oliva N., IRRI [International Rice Research Institute] (PHL)
• Datta K., IRRI [International Rice Research Institute] (PHL)
• Frutos Roger, CIRAD-EMVT-UMR TRYPANOSOMES (FRA)
• Datta S.K., IRRI [International Rice Research Institute] (PHL)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/542104/)

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