Risterucci Ange-Marie, Hippolyte Isabelle, Caig Vanessa, Carling J., Evers Margaret, Uszynski Grzegorz, Wenzl Peter, Xia L., Huttner Eric, Glaszmann Jean-Christophe, Kilian Andrzej.
2006. DArT, a whole genome profiling method for high-troughput genotyping of Musa.
In : Plant Genomics European Meetings, Venice, 2006
Résumé : Genetic analysis of adaptation in plants is of primary interest in the case of crops as well as natural populations, for the sake of sustainable agriculture and ecological resource management. Both accurate assessment of the right phenotypic features and fast and affordable high throughput genotyping are necessary. If we manage to make the latter no longer limiting, we open a wider range of opportunities to produce valuable genetic information using materials with phenotypic description derived from diverse and rich experiments and protocols. The sequential nature of gel-based markers entails a limitation in throughput. Many marker systems such as SNP and SSR depend on sequence information. Diversity Arrays Technology (DArT) circumvents these limitations and enables high-throughput genome profiling at low cost even for crops with no sequence information available. Musa is one of the world's most important crop genera with 73 million tons of banana and 33 million tons of plantain fruits produced in 2005. It features several genomes in various combinations. We have now established two libraries of 6,000 DArT clones using a complexity reduction method based on restriction enzymes Pstl as a primary cutter and Taql or BstNl as a secondary cutter. Between 700 and 800 markers were identified in each of these libraries. Initial analysis of 187 diverse banana and plantain accessions showed that while the majority of the markers separated the A and the B genome, the array also resolves diversity within each genome. This current set of arrays will be applied to extend the current genetic map of banana, explore applications of DArTs for ordering BAC libraries and for anchoring the genetic and physical maps. (Texte intégral)
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Risterucci Ange-Marie, CIRAD-AMIS-UMR PIA (FRA)
- Hippolyte Isabelle, CIRAD-AMIS-UMR PIA (FRA)
- Caig Vanessa, Diversity Arrays Technology (AUS)
- Carling J., Diversity Arrays Technology (AUS)
- Evers Margaret, Diversity Arrays Technology (AUS)
- Uszynski Grzegorz, Diversity Arrays Technology (AUS)
- Wenzl Peter, Diversity Arrays Technology (AUS)
- Xia L., Diversity Arrays Technology (AUS)
- Huttner Eric, Diversity Arrays Technology (AUS)
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Glaszmann Jean-Christophe, CIRAD-AMIS-UMR PIA (FRA)
ORCID: 0000-0001-9918-875X
- Kilian Andrzej, Diversity Arrays Technology (AUS)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/542335/)
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