Vivien Eric, Megessier Sandrine, Pieretti Isabelle, Cociancich Stéphane, Frutos Roger, Gabriel Dean W., Rott Philippe, Royer Monique. 2005. Xanthomonas albilineans HtpG is required for biosynthesis of the antibiotic and phytotoxin albicidin. FEMS Microbiology Letters, 251 (1) : 81-89.
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Résumé : Xanthomonas albilineans, the causal agent of leaf scald disease of sugarcane, produces a highly potent polyketide-peptide antibiotic and phytotoxin called albicidin. Previous studies established the involvement of a large cluster of genes in the biosynthesis of this toxin. We report here the sub-cloning and sequencing of an additional gene outside of the main cluster and essential for albicidin biosynthesis. This gene encodes a 634-amino-acid protein that shows high identity with the Escherichia coli heat shock protein HtpG. Complementation studies of X. albilineans Tox_ mutants confirmed the requirement of htpG for albicidin biosynthesis and revealed functional interchangeability between E. coli and X. albilineans htpG genes. HtpG was co-localised with albicidin in the cellular membrane, i.e., the cellular fraction where the toxin is most probably biosynthesised. Here we show the requirement of an HtpG protein for the biosynthesis of a polyketide-peptide antibiotic.
Classification Agris : H20 - Maladies des plantes
Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique
Auteurs et affiliations
- Vivien Eric, UM2 (FRA)
- Megessier Sandrine
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Pieretti Isabelle, CIRAD-AMIS-UMR BGPI (FRA)
ORCID: 0009-0003-3498-1230
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Cociancich Stéphane, CIRAD-AMIS-UMR BGPI (FRA) ORCID: 0000-0001-8601-3598
- Frutos Roger, CIRAD-EMVT-UMR TRYPANOSOMES (FRA)
- Gabriel Dean W., University of Florida (USA)
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Rott Philippe, CIRAD-AMIS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159
- Royer Monique, CIRAD-AMIS-UMR BGPI (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/542377/)
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