Virulence and pathogenicity patterns of Trypanosoma brucei gambiense field isolates in experimentally infected mouse : Differences in host immune response modulation by secretome and proteomics

Holzmuller Philippe, Biron David George, Courtois Pierrette, Koffi Mathurin, Daulouède Sylvie, Solano Philippe, Cuny Gérard, Vincendeau Philippe, Jamonneau Vincent. 2008. Virulence and pathogenicity patterns of Trypanosoma brucei gambiense field isolates in experimentally infected mouse : Differences in host immune response modulation by secretome and proteomics. Microbes and Infection, 10 (1) : 79-86.

Résumé : Human African trypanosomiasis is characterised by an important clinical diversity. Although Trypanosoma brucei gambiense field stocks isolated from patients in the same focus did not exhibit apparent genetic variability, they showed marked differences in terms of virulence (capacity to multiply inside a host) and pathogenicity (ability of producing mortality) in experimental murine infections. Two strains exhibiting opposite pathogenic and virulence properties in mouse were further investigated through their host-parasite interactions. In vitro, parasite bloodstream forms or soluble factors (or secretome) from both strains induced macrophage arginase as a function of their virulence. Arginase expression, a hallmark of macrophage alternative activation pathway, favours trypanosome bloodstream forms development. Moreover, a comparative proteomic study of the trypanosome stocks' secretomes evidenced both a differential expression of common molecules and the existence of stock specific molecules. This highlighted the potential involvement of the differential expression of the same genome in the diverse infectious properties of trypanosomes.

Mots-clés Agrovoc : Trypanosoma brucei, pouvoir pathogène, épidémiologie, immunologie, expression des gènes, hôte, virulence

Mots-clés complémentaires : Protéomique

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Holzmuller Philippe, CIRAD-BIOS-UMR TRYPANOSOMES (FRA) ORCID: 0000-0002-8919-9081
• Biron David George
• Courtois Pierrette, Université de Bordeaux II (FRA)
• Koffi Mathurin, CIRDES (BFA)
• Daulouède Sylvie, Université de Bordeaux II (FRA)
• Solano Philippe, IRD (BFA)
• Cuny Gérard, IRD (FRA)
• Vincendeau Philippe, Université de Bordeaux II (FRA)
• Jamonneau Vincent, CIRDES (BFA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/544180/)

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