Identification of early molecular markers associated with the mantled phenotype in micropropagated oil palms by subtractive PCR and cDNA array analysis

Beulé Thierry, Marguerettaz Mélanie, Morcillo Fabienne, Fuentes Ivanna, Singh Rajinder, Tregear James. 2007. Identification of early molecular markers associated with the mantled phenotype in micropropagated oil palms by subtractive PCR and cDNA array analysis. In : MPOB International Palm Oil Congress (PIPOC 2007), 26 to 30 August 2007, Kuala Lumpur, Malaysia. MPOB. s.l. : s.n., 6 p. MPOB International Palm Oil Congress, Kuala Lumpur, Malaisie, 26 Août 2007/30 Août 2007.

Résumé : Tissue culture of oil palm induces somaclonal variation. The main one, known as the mantled abnormality, displays homeotic floral modifications and is associated with a lower oil yield. To detect abnormal cell lines as early as possible during the tissue culture process, studies to identify molecular markers have been carried out. Given the epigenetic nature of the mantled phenotype, differential gene expression has been focused upon rather than genome structure. We investigated gene expression patterns at the transcriptome level for three developmental stages: embryogenic cell suspensions, somatic embryos and leafy shoots. In the first step, the technique of subtractive PCR (SSH) was used to construct six cDNA libraries from variant and normal plant materials allowing enrichment for cDNA corresponding to genes which are up- or down-regulated in association with clonal conformity. In the second step, transcript abundance was monitored by macroarray hybridization. A range of genes altered in their expression in abnormal tissues has been identified and a number of which have been validated by RT-PCR. This approach will provide insights into the biological processes involved in the generation of the mantle phenotype and the identified markers should lead to the development of a clonal conformity test.

Classification Agris : F30 - Génétique et amélioration des plantes
F02 - Multiplication végétative des plantes

Auteurs et affiliations

• Beulé Thierry, CIRAD-BIOS-UMR DAP (FRA)
• Marguerettaz Mélanie, CIRAD-BIOS-UMR BGPI (FRA)
• Morcillo Fabienne, CIRAD-BIOS-UMR DAP (FRA)
• Fuentes Ivanna, CIRAD-BIOS-UMR DAP (FRA)
• Singh Rajinder, MPOB (MYS)
• Tregear James, IRD (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/545881/)

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