A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm

Iskra Caruana Marie-Line, Galzi Serge, Laboureau Nathalie. 2008. A reliable IC One-step RT-PCR method for the detection of BBrMV to ensure safe exchange of Musa germplasm. Journal of Virological Methods, 153 (2) : 223-231.

https://doi.org/10.1016/j.jviromet.2008.06.028

Article de revue ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_546691.pdf
Télécharger (1MB)

Résumé : An immunocapture (IC) One-step RT-PCR assay was developed to improve the detection of Banana bract mosaic virus (BBrMV) in single and bulked samples of banana plants. In this paper, an atypical strain of BBrMV was described, the BBrMV "Ref" strain, and we showed that detection with available BBrMV tools using ELISA and RT-PCR approacheswas not reliable. Primer sets Bract N1/NR and N2/NR specific to BBrMV were designed and used in RT-PCR and IC-RT-PCR assays with two commercial kits that allow the RT and the PCR reactions to take place simultaneously in the same tube. The new assay enabled detection of BBrMV in leaf extract diluted up to 1×10-10 and in bulked samples of 10 plants, and was proposed as a new international standard to index BBrMV.

Mots-clés Agrovoc : Musa, virus des végétaux, PCR, Test ELISA, diagnostic, germoplasme

Mots-clés complémentaires : Virus de la mosaïque des bractées

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations