Study of bovine trypanotolerance by whole transcriptome analysis: Toward identification of the involved genes

Berthier David, Chantal Isabelle, Thevenon Sophie, Sakande Hassane, Maillard Jean-Charles, Bengaly Zakaria, Piquemal David, Marti Jacques, Cuny Gérard. 2008. Study of bovine trypanotolerance by whole transcriptome analysis: Toward identification of the involved genes. Annals of the New York Academy of Sciences, 1149 : 71-76. Biennial Conference of the Society for Tropical Veterinary Medicine. 9, Mexico, Mexique, 17 Juin 2007/22 Juin 2007.

https://doi.org/10.1196/annals.1428.062

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Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Histoire et philosophie des sciences; Histoire, histoire de l'art, archéologie; Psychologie-éthologie-ergonomie

Résumé : African trypanosomiases are parasitic diseases transmitted by tse-tse flies, considered as themain sanitary obstacle to animal production development in sub-Saharan Africa. However, if trypanosomiases have dramatic consequences on zebu (Bos indicus) populations, they have a weaker impact on the western African taurine (Bos taurus), which is known to be naturally tolerant to trypanosome infection. Mechanisms governing this trypanotolerant trait are still poorly understood, but today, recent postgenomic biotechnologies, such as the SAGE technique (serial analysis of gene expression) allow us to explore the full transcriptome. Twelve SAGE libraries were constructed from two trypanotolerant animals (N'Dama and Baoul´e) and one susceptible species of cattle (the Sudanese zebu) during an experimental Trypanosoma congolense infection; 43,458 different tags were obtained at several particular points during the infection (before infection, at the maximum of parasitemia, the maximum of anemia, and at the end of the experiment after value normalization). Bioinformatics analyses highlighted some interesting gene variations with respect to the trypanotolerance status of the animal.

Mots-clés Agrovoc : Trypanosoma congolense, tolérance, bovin, expression des gènes, arn messager, technique analytique, banque de gènes, identification

Mots-clés géographiques Agrovoc : Burkina Faso

Classification Agris : L10 - Génétique et amélioration des animaux
L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

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