Comparison of GUS activity in self-rooting and budded primary trans/ormant plants in Hevea brasiliensis

Lardet Ludovic, Benistant Elise, Leclercq Julie, Martin Florence, Oliver Gérald, Montoro Pascal. 2008. Comparison of GUS activity in self-rooting and budded primary trans/ormant plants in Hevea brasiliensis. In : IRRDB Natural Rubber Conference, Kuala Lumpur, Malaisie, 13-14 October 2008. s.l. : s.n., 6 p. IRRDB Natural Rubber Conference, Kuala Lumpur, Malaisie, 13 Octobre 2008/14 Octobre 2008.

Résumé : Successful both somatic embryogenesis and Agrobacterium tumefaciens-mediated genetic transformation systems have been developed in PB260 clone. Several transgenic callus lines and plants expressing two reporter genes (gusA and/or GFP) and CuZnSOD gene driven by the CAMV 35S promoter are respectively maintained by cryopreservation and in greenhouse. Functional genomic analysis requires homogenous population of plants for further characterization. However, the primary transformant plants are considered unstable leading to a variation in transgene expression. With regards to this epigenetic control, the variability of the beta-Dglucuronidase activity has been assessed by fluorimetric assays in primary transformant plants both self-rooting and budded state. Our preliminary results show a relative homogeneity of GUS activity in the population of self-rooting primary transformant plants obtained directly by somatic embryogenesis. After green budding, a decrease in GUS activity is recorded leading to a higher variability. The effects of budding, rootstock, rootstock/scion interaction, quality and position of the scion are discussed.

Mots-clés Agrovoc : Hevea brasiliensis

Classification Agris : F30 - Génétique et amélioration des plantes
F02 - Multiplication végétative des plantes

Auteurs et affiliations

• Lardet Ludovic, CIRAD-BIOS-UMR DAP (FRA)
• Benistant Elise
• Leclercq Julie, CIRAD-BIOS-UMR DAP (FRA) ORCID: 0000-0001-8110-3762
• Martin Florence, CIRAD-BIOS-UMR DAP (FRA)
• Oliver Gérald, CIRAD-BIOS-UMR DAP (FRA)
• Montoro Pascal, CIRAD-BIOS-UMR DAP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/547537/)

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