Effect of growth regulators on microspore embryogenesis in coconut anthers

Perera Prasanthi I.P., Yakandawala Deepthi M.D., Hocher Valérie, Verdeil Jean-Luc, Weerakoon L. Kaushalya. 2009. Effect of growth regulators on microspore embryogenesis in coconut anthers. Plant Cell, Tissue and Organ Culture, 96 : 171-180.

https://doi.org/10.1007/s11240-008-9473-y

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Quartile : Q2, Sujet : PLANT SCIENCES / Quartile : Q3, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Résumé : The effect of growth regulators on induction of androgenesis in coconut was investigated using seven different growth regulators at various concentrations and combinations. Three auxins (1-naphthalene acetic acid-NAA, indoleacetic acid-IAA, picloram) and three cytokinins (2-isopentyl adenine-2-iP, kinetin, zeatin) were tested either alone or in combination with 2,4-dichlorophenoxyacetic acid (2,4-D), using modified Eeuwens Y3 liquid medium as the basal medium. Among the tested auxins, 100 [mu]M NAA in combination with 100 [mu]M 2,4-D enhanced the production of calli/embryos (123) whereas IAA and picloram showed negative and detrimental effects, respectively, for androgenesis induction over 100 lM 2,4- D alone. Kinetin and 2-iP enhanced the production of calli/ embryos when 100 [mu]M 2,4-D was present in the culture medium. Both cytokinins at 10 [mu]M yielded the highest frequencies of embryos (113 and 93, respectively) whereas zeatin (1 or 2.5 [mu]M) had no impact on microspore embryogenesis. When calli/embryos (produced from different treatments in different experiments) were subcultured in somatic embryo induction medium (Y3 medium containing 66 [mu]M 2,4-D), followed by maturation medium (Y3 medium without growth regulators) and germination medium (Y3 medium containing 5 [mu]M-6-benzyladenine- BA and 0.35 [mu]M gibberellic acid-GA3), plantlets were regenerated at low frequencies (in most treatments ranging from 0% to 7%).

Mots-clés Agrovoc : Cocos nucifera

Mots-clés géographiques Agrovoc : Sri Lanka

Classification Agris : F60 - Physiologie et biochimie végétale
F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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