Establishment of a cryopreservation process for long-term storage of embryogenic calli of Hevea brasiliensis (Müll. Arg.).

Lardet Ludovic, Martin Florence, Chapuset Thierry, Carron Marc-Philippe, Montoro Pascal. 2006. Establishment of a cryopreservation process for long-term storage of embryogenic calli of Hevea brasiliensis (Müll. Arg.).. In : International Natural Rubber Conference, November 13-15, 2006, Ho Chi Minh, Vietnam: Preprints of papers. Mai Van Son (ed.), Nugyen Ngoc Bich (ed.), Tong Viet Thinh (ed.). IRRI, RRIV. Ho Chi Minh City : Rubber Research Institute of Vietnam, 684-690. International Natural Rubber Conference, Ho Chi Minh, Viet Nam, 13 Novembre 2006/14 Novembre 2006.

Résumé : A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The technique included a callus pre-culture on a maintenance medium containing 1 mM CaCl2 prior to cryopreservation to promote postthaw callus growth recovery. Application of this cryopreservation technique on a sample of 39 callus lines, showed a high percentage of post-thaw lines growth recovery and subsequent maintenance of embryogenic and regeneration competences. Today, all the lines obtained including transformed lines were cryopreserved using this procedure. Data base has been developed for management of cryopreserved stocks.

Mots-clés Agrovoc : Hevea brasiliensis

Classification Agris : F02 - Multiplication végétative des plantes
F62 - Physiologie végétale - Croissance et développement

Auteurs et affiliations

• Lardet Ludovic, CIRAD-CP-UMR BEPC (FRA)
• Martin Florence, CIRAD-BIOS-UMR DAP (FRA)
• Chapuset Thierry, CIRAD-CP-UPR Systèmes de pérennes (FRA)
• Carron Marc-Philippe, CIRAD-CP-UMR BEPC (FRA)
• Montoro Pascal, CIRAD-CP-UMR BEPC (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/548921/)

Métriques

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