Isolation and characterization of three members of the multigenic family encoding ACC oxidase from H. brasiliensis during plant development : [Draft]

Leclercq Julie, Sumarmadji, Rio Maryannick, Alemanno Laurence, Montoro Pascal. 2005. Isolation and characterization of three members of the multigenic family encoding ACC oxidase from H. brasiliensis during plant development : [Draft]. In : IRRDB Natural Rubber Conference, Cochin, Keral, India, 7-8 November 2005. IRRDB. s.l. : s.n., 1-5. IRRDB Natural Rubber Conference, Kerala, Inde, 7 Novembre 2005/8 Novembre 2005.

Résumé : Applying Ethephon, an ethylene releaser, to increase rubber production in H. brasiliensis has been practised for a long time. The effect of ethylene on latex production has been amply described, notably for lengthening latex flow and its regeneration. Nevertheless, little is known about the expression of genes involved in ethylene biosynthesis and response to ethylene. In this paper, we isolated and characterized genes encoding ACC oxidase, a key enzyme in ethylene biosynthesis in the plant. We then studied the effect of ethylene stimulation on the expression of ACO genes in various tissues during plant development. Three members of the ACO multigenic family were isolated from a bark cDNA library with RACE technology: HbACO-H4, HbACO-H5, and HbACO-O48. Full length cDNA sequences encoded for peptides of 318, 315, and 318 amino acids respectively for these 3 members, which had 79 to 92% protein identity and 75 to 86% nucleotide homology between them. Two genomic sequences were isolated: HbACO-H4, which was 1504 bp long and consisted of 2 introns and 3 exons, while Hb-ACO-H5 was 1456 bp long and consisted of 3 introns and 4 exons. These three genes were differentially expressed in different plant organs in response to ethylene stimulation.

Mots-clés Agrovoc : Hevea brasiliensis

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement

Auteurs et affiliations

• Leclercq Julie, CIRAD-CP-UMR BEPC (FRA) ORCID: 0000-0001-8110-3762
• Sumarmadji, IRRI [Indonesian Rubber Research Institute] (IDN)
• Rio Maryannick, CIRAD-CP-UMR BEPC (FRA) ORCID: 0000-0002-4573-1371
• Alemanno Laurence, CIRAD-CP-UMR BEPC (FRA)
• Montoro Pascal, CIRAD-CP-UMR BEPC (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/548933/)

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