Different ways of integrating in vitro culture in Hevea planting material propagation

Carron Marc-Philippe, Lardet Ludovic, Montoro Pascal. 2005. Different ways of integrating in vitro culture in Hevea planting material propagation. In : International Natural Rubber Conference : preprints of conference, 6-8 November 2005, Cochin, India. Kottayam : Rubber Board, 79-82. International Natural Rubber Conference, Cochin, Inde, 6 Novembre 2005/8 Novembre 2005.

Résumé : Somatic embryos and shoots were obtained from cotyledon derived cultures of Hevea brasiliensis. Embryogenic callus were induced from cotyledon sections on improved MS medium with 2 mg/L 2,4-D and 0.5 mg/L GA . Embryo development could be promoted on MS medium containing 4.0~6.0 mg/L KT +0.2 m3g/L IAA or 1.0 mg/L ZT +0.5 mg/L GA +0.2 mg/L NAA. Each of the most explants could develop 3-5 mature embryos, the highest oie had induced 25 mature embryos. 15.57 percent of mature embryos developed into normal plantlets. This regeneration system may be useful for creating rubber rootstock clones. Its polyembryo character will be beneficial to improve somatic embryo induction frequency ìn Hevea brasiliensis, which is one of the main obstacles in rubber tissue culture.

Mots-clés Agrovoc : Hevea brasiliensis

Mots-clés géographiques Agrovoc : Thaïlande, Nigéria, Côte d'Ivoire

Classification Agris : F02 - Multiplication végétative des plantes

Auteurs et affiliations

• Carron Marc-Philippe, CIRAD-CP-UMR BEPC (FRA)
• Lardet Ludovic, CIRAD-CP-UMR BEPC (FRA)
• Montoro Pascal, CIRAD-CP-UMR BEPC (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/548984/)

Métriques

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