Arnau Gemma, Némorin Alice, Maledon Erick, Abraham Kuttolamadathil. 2009. Revision of ploidy status of Dioscorea alata L. (Dioscoreaceae) by cytogenetic and microsatellite segregation analysis. Theoretical and Applied Genetics, 118 (7) : 1239-1249.
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Quartile : Q1, Sujet : AGRONOMY / Quartile : Outlier, Sujet : HORTICULTURE / Quartile : Q1, Sujet : PLANT SCIENCES / Quartile : Q2, Sujet : GENETICS & HEREDITY
Résumé : Dioscorea alata is a polyploid species with several ploidy levels and its basic chromosome number has been considered by most authors to be x = 10. Standard chromosome counting and flow cytometry analysis were used to determine the chromosome number of 110 D. alata accessions of the CIRAD germplasm collection. The results revealed that 76% of accessions have 2n = 40 chromosomes, 7% have 2n = 60 chromosomes and 17% have 2n = 80 chromosomes. Progenies were produced from 2n = 40 types of D. alata and the segregation patterns of six microsatellite markers in four different progenies were analysed. The Bayesian method was used to test for diploid versus tetraploid (allo- and autotetraploid) modes of inheritance. The results provided the genetic evidence to establish the diploidy of plants with 2n = 40 chromosomes and to support the hypothesis that plants with 2n = 40, 60 and 80 chromosomes are diploids, triploids and tetraploids, respectively, and that the basic chromosome number of D. alata is x = 20. The findings obtained in the present study are significant for effective breeding programs, genetic diversity analysis and elucidation of the phylogeny and the species origin of D. alata.
Mots-clés Agrovoc : Dioscorea alata, polyploïdie, microsatellite, cytogénétique
Mots-clés géographiques Agrovoc : Antilles françaises, Guadeloupe, France
Classification Agris : F30 - Génétique et amélioration des plantes
Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique
Auteurs et affiliations
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Arnau Gemma, CIRAD-BIOS-UPR Multiplication végétative (GLP)
ORCID: 0000-0003-0714-6830
- Némorin Alice, CIRAD-BIOS-UPR Multiplication végétative (GLP)
- Maledon Erick, CIRAD-BIOS-UPR Multiplication végétative (GLP)
- Abraham Kuttolamadathil, CTCRI (IND)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/548994/)
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