Evaluation of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus in sera of domestic ruminants in France

Cetre-Sossah Catherine, Billecocq Agnès, Lancelot Renaud, Defernez Cédric, Favre Jacques, Bouloy Michèle, Martinez Dominique, Albina Emmanuel. 2009. Evaluation of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus in sera of domestic ruminants in France. Preventive Veterinary Medicine, 90 (1-2) : 146-149.

https://doi.org/10.1016/j.prevetmed.2009.03.011

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Quartile : Q1, Sujet : VETERINARY SCIENCES

Résumé : This paper describes the sensitivity and specificity of a commercial competitive ELISA for the detection of antibodies to Rift Valley fever virus (RVFV) from sera of French domestic ruminants. Field samples were collected from mainland France for the known-negative sera (cattle = 191, goats = 119, sheep = 192) and from ruminants of a French overseas territory (Mayotte) for the known-positive sera. A cut-off value of 43% was determined for all species, achieving a sensitivity and specificity of 100% and a concordance of 100% with the species-specific threshold recommended by the manufacturer. Our results demonstrate that this ELISA may be a suitable diagnostic tool for disease surveillance programmes and import/export veterinary certification of French cattle, goats and sheep.

Mots-clés Agrovoc : ruminant, Virus de la fièvre de la vallée du Rift, Test ELISA, fièvre de la Vallée du Rift

Mots-clés géographiques Agrovoc : France

Classification Agris : L73 - Maladies des animaux

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

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