Genetic determination and temperature effects on turbot Scophthalmus maximus sex differentiation : An investigation using steroid sex-inverted males and females

Haffray Pierrick, Lebègue Elisabeth, Jeu Sylvie, Guennoc Michel, Guiguen Yann, Baroiller Jean-François, Fostier Alexis. 2009. Genetic determination and temperature effects on turbot Scophthalmus maximus sex differentiation : An investigation using steroid sex-inverted males and females. Aquaculture, 294 (1-2) : 30-36.

Quartile : Q1, Sujet : FISHERIES / Quartile : Q2, Sujet : MARINE & FRESHWATER BIOLOGY

Résumé : The relative roles of genetic (GSD) and temperature sex determination (TSD) were investigated in turbot, Scophthalmus maximus, with the objective of producing all-female stocks for aquaculture. Androgen treated males (ATM) and oestrogen treated females (OTF) were produced after testing various 17?-methyltestosterone or 17?-oestradiol treatments delivered orally (3 or 5 mg steroids/kg food) from 35 days postfertilization (dpf) during either 500 or 700 degree-days respectively. GSD was analyzed by checking the sex ratio of families (n=33) obtained by crossing control parents with other control parents, either ATM or OTF. TSD was investigated on 8 families reared at three temperatures (15 °C, 17 °C or 24 °C) from weaning to 100 dpf. All the steroid treatments were efficient in producing either 100% male or 100% female populations demonstrating the production of androgen sex-inversed males and oestrogen sex-inversed females. The results of the GSD analysis suggested that most of the data fit to a ZZ/ZW model of sex determination. However, some crosses deviated from this model and other minor factors may be involved. Interestingly, minor effects of temperature and a limited but significant interaction between family and temperature have been observed. In conclusion, the present data suggest that a major ZZ/ZW genetic model is involved in turbot sex determination. Unexpected sex ratios deviating from this model are discussed, including a possible minor effect of environmental factors, such as temperature.

Mots-clés Agrovoc : Scophthalmus maximus, détermination du sexe, différenciation sexuelle, stéroïde, température, génétique

Mots-clés géographiques Agrovoc : Pays de la Loire, France

Classification Agris : L53 - Physiologie animale - Reproduction
M12 - Production de l'aquaculture

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Haffray Pierrick, SYSAAF (FRA)
• Lebègue Elisabeth
• Jeu Sylvie
• Guennoc Michel
• Guiguen Yann, INRA (FRA)
• Baroiller Jean-François, CIRAD-PERSYST-UPR Aquaculture (FRA) ORCID: 0000-0002-0992-1725
• Fostier Alexis, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/549966/)

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