Associating candidate defense genes with quantitative resistance to rice blast : [P91]

Carrillo G., Wu J., Liu B., Sugiyama N., Ona I., Variar M., Bhatt J.C., Javier E., Goodwin P., Courtois Brigitte, et al.. 2005. Associating candidate defense genes with quantitative resistance to rice blast : [P91]. In : 5th International Rice Genetics Symposium (A platform for exploring developments in rice genetics and their applications) and 3rd International Symposium of Rice Functional Genomics. Abstract. IRRI. Manila : IRRI, Résumé, p. 99. International Rice Genetics Symposium. 5, Manille, Philippines, 19 November 2005/23 November 2005.

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Abstract : We used the candidate gene (CG) approach to accumulate different mechanisms involved in quantitative resistance to rice blast in 84 selected lines derived from advanced backcross populations of Vandana/Moroberekan. Using sequence information and molecular markers from the rice genome, we designed gene-based markers and identified SSRs within CGs. These markers were used to conduct a genome scan to determine the proportion of CG alleles from Moroberekan in 108 introgression lines (84 selected for blast resistance and 24 for drought tolerance). Single-marker analysis identified three CGs significantly correlated with blast resistance. Oxalate oxidase and peroxidase were correlated with yield under blast as well as with panicle blast in Almora, a blast hotspot in India. Thaumatin is correlated with neck blast incidence in Cavinti, Philippines. Significant two-gene interactions (P<0.001)were observed in seedling blast (HSP90 x thaumatin and thaumatin x oxalate oxidase) and neck blast (chitinase x thaumatin and thaumatin x oxalate oxidase-like protein). Of the lines selected for blast resistance, 23 yielded more than Vandana under natural drought stress. These include two lines (IR78221-19-6-7-B-B and IR78221-19-6-99-B-B) that are also high-yielding under high blast pressure in Almora. IR78221-19-6- 7-B-B has alleles of oxalate oxidase, peroxidase, and HSP90 from Moroberekan. IR78221-19-6-7-B-B has only oxalate oxidase allele from Moroberekan. We are testing if additional candidate defense genes are responsible for the observed resistance in these lines. By combining multi-location tests and molecular analysis, we have identified gene-based markers strongly associated with partial blast resistance. We are now in a position to combine blast resistance with drought tolerance in advanced breeding populations for a target environment in India. (Résumé d'auteur)

Mots-clés Agrovoc : Oryza

Mots-clés géographiques Agrovoc : Inde, Philippines

Classification Agris : F30 - Plant genetics and breeding
H20 - Plant diseases
H50 - Miscellaneous plant disorders

Auteurs et affiliations

  • Carrillo G., IRRI (PHL)
  • Wu J., IRRI (PHL)
  • Liu B., IRRI (PHL)
  • Sugiyama N., IRRI (PHL)
  • Ona I., IRRI (PHL)
  • Variar M., CRURRS (IND)
  • Bhatt J.C., ICAR (IND)
  • Javier E., IRRI (PHL)
  • Goodwin P., University of Guelph (CAN)
  • Courtois Brigitte, CIRAD-AMIS-UMR PIA (FRA) ORCID: 0000-0003-2118-7102
  • et al.

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