A new semi-selective medium for isolation of Xanthomonas axonopodis pv. dieffenbachiae, the etiological agent of anthurium bacterial blight

Laurent Philippe, Chabirand Aude, Jouen Emmanuel, Robène-Soustrade Isabelle, Gagnevin Lionel, Hostachy Bruno, Pruvost Olivier. 2009. A new semi-selective medium for isolation of Xanthomonas axonopodis pv. dieffenbachiae, the etiological agent of anthurium bacterial blight. Letters in Applied Microbiology, 49 (2) : 210-216.

Quartile : Q3, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q3, Sujet : MICROBIOLOGY

Résumé : Aims: Xanthomonas axonopodis pv. dieffenbachiae causes anthurium blight, which is regarded as the most threatening disease for the anthurium industry worldwide. The bacterium is listed as a quarantine pathogen in several regions, including Europe. We evaluated the use of Neomycin-Cephalexin-Trimethoprime-pivMecillinam 4 (NCTM4) medium for its isolation. Methods and Results: A total of 104 bacterial strains were inoculated onto NCTM4 and on the previously published Cellobiose-Starch (CS) and Esculin-Trehalose (ET) media. The strain collection included: the anthurium blight pathogen, Xanthomonas strains, for which false positive results are known to occur using serological identification-tests; other bacterial pathogens of anthurium; and representatives of bacteria that are commonly present in the anthurium phyllosphere. Media were evaluated following the ISO 16140 protocol for the validation of alternative methods. Conclusion: Growth of the anthurium blight pathogen was better on NCTM4 and ET media than on CS. NCTM4 provided a better repeatability. It also displayed a lower rate of false positive and false negative results when the pathogen was isolated from plant extracts. Significance and Impact of the Study: This study will lead to improved isolation protocols of the anthurium blight in official procedures. NCTM4 medium could also favourably be used in studies, which aim to further understanding of the biology and epidemiology of this pathogen.

Mots-clés Agrovoc : Xanthomonas, Anthurium, Xanthomonas axonopodis

Mots-clés géographiques Agrovoc : La Réunion, France

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Laurent Philippe
• Chabirand Aude, Ministère de l'alimentation, de l'agriculture et de la pêche (France) (REU)
• Jouen Emmanuel, Université de la Réunion (REU)
• Robène-Soustrade Isabelle, CIRAD-BIOS-UMR PVBMT (REU)
• Gagnevin Lionel, CIRAD-BIOS-UMR PVBMT (REU)
• Hostachy Bruno, Ministère de l'alimentation, de l'agriculture et de la pêche (France) (REU)
• Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/550557/)

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