Biphasic haustorial differentiation of coffee rust (Hemileia vastatrix race II) associated with defence responses in resistant and susceptible coffee cultivars

Ramiro D.A., Escoute Jacques, Petitot Anne-Sophie, Nicole Michel, Maluf M.P., Fernandez Diana. 2009. Biphasic haustorial differentiation of coffee rust (Hemileia vastatrix race II) associated with defence responses in resistant and susceptible coffee cultivars. Plant Pathology, 58 (5) : 944-955.

Quartile : Q1, Sujet : AGRONOMY / Quartile : Q1, Sujet : PLANT SCIENCES

Résumé : The objective of this study was to assess whether defence responses in coffee (Coffea arabica) were linked to a specific developmental stage of the rust fungus Hemileia vastatrix. Histological observations in compatible and incompatible highyielding Brazilian coffee cultivars showed that the fungus produced 'pioneer' haustoria in adjacent and subsidiary stomatal cells soon after entering the stomata, followed by later developed 'secondary haustoria' which invade mesophyll cells. In the incompatible interaction between Race II and cv. Tupi, a strong and transient H2O2 generation at infection sites was detected at 39 h post inoculation (hpi) during secondary haustoria formation. In addition, clear-cut differences in defence gene expression between compatible and incompatible interactions were only observed during the secondary haustoria formation. Transcripts of the pathogenesis-related (PR) genes CaPR1b and CaPR10 accumulated to maximal levels at 39 hpi (38- and 86-fold, respectively) in the incompatible interaction, but stayed at low levels in the compatible interaction. In contrast, the CaWRKY1 gene and the CaRLK gene were only induced in the susceptible cultivar. These results indicated that the specific resistance of cv. Tupi was expressed after differentiation of the H. vastatrix secondary haustoria. Analysis showed no evidence of specific recognition of coffee rust at the pioneer haustoria stage, suggesting that haustoria components are not recognized by, or not secreted into, the subsidiary and adjacent cells of the stomata. Additionally, the present study provides new insights into the colonization process of the coffee rust fungus.

Mots-clés Agrovoc : Coffea arabica, Hemileia vastatrix

Mots-clés géographiques Agrovoc : Brésil

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Ramiro D.A., IRD (FRA)
• Escoute Jacques, CIRAD-BIOS-UMR DAP (FRA)
• Petitot Anne-Sophie, EMBRAPA (BRA)
• Nicole Michel, IRD (FRA)
• Maluf M.P., EMBRAPA (BRA)
• Fernandez Diana, IRD (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/551904/)

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