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Identification of total and differentially expressed excreted-secreted proteins from Trypanosoma congolense strains exhibiting different virulence and pathogenicity

Grébaut Pascal, Chuchana Paul, Brizard Jean-Paul, Demettre Edith, Seveno Marc, Bossard Géraldine, Jouin Patrick, Vincendeau Philippe, Bengaly Zakaria, Boulangé Alain, Cuny Gérard, Holzmuller Philippe. 2009. Identification of total and differentially expressed excreted-secreted proteins from Trypanosoma congolense strains exhibiting different virulence and pathogenicity. International Journal for Parasitology, 39 (10) : pp. 1137-1150.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : PARASITOLOGY

Abstract : Animal trypanosomosis is a major constraint to livestock productivity in the tropics and has a significant impact on the life of millions of people globally (mainly in Africa, South America and south-east Asia). In Africa, the disease in livestock is caused mainly by Trypanosoma congolense, Trypanosoma vivax, Trypanosoma evansi and Trypanosoma brucei brucei. The extracellular position of trypanosomes in the bloodstream of their host requires consideration of both the parasite and its naturally excreted-secreted factors (secretome) in the course of pathophysiological processes. We therefore developed and standardised a method to produce purified proteomes and secretomes of African trypanosomes. In this study, two strains of T. congolense exhibiting opposite properties of both virulence and pathogenicity were further investigated through their secretome expression and its involvement in host-parasite interactions. We used a combined proteomic approach (one-dimensional SDS-PAGE and two-dimensional differential in-gel electrophoresis coupled to mass spectrometry) to characterise the whole and differentially expressed protein contents of secretomes. The molecular identification of differentially expressed trypanosome molecules and their correlation with either the virulence process or pathogenicity are discussed with regard to their potential as new diagnostic or therapeutic tools against animal trypanosomosis. (Résumé d'auteur)

Mots-clés Agrovoc : Trypanosoma congolense

Mots-clés géographiques Agrovoc : Burkina Faso

Mots-clés complémentaires : Protéomique

Classification Agris : L73 - Animal diseases

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Grébaut Pascal, IRD (FRA)
  • Chuchana Paul, IRD (FRA)
  • Brizard Jean-Paul, CNRS (FRA)
  • Demettre Edith, UM1 (FRA)
  • Seveno Marc, UM1 (FRA)
  • Bossard Géraldine, CIRAD-BIOS-UMR TRYPANOSOMES (FRA)
  • Jouin Patrick, UM1 (FRA)
  • Vincendeau Philippe, Université de Bordeaux II (FRA)
  • Bengaly Zakaria, CIRDES (BFA)
  • Boulangé Alain, CIRAD-BIOS-UMR TRYPANOSOMES (ZAF)
  • Cuny Gérard, IRD (FRA)
  • Holzmuller Philippe, CIRAD-BIOS-UMR TRYPANOSOMES (FRA) ORCID: 0000-0002-8919-9081

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/554918/)

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