Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11

Chan Salum Thais Fabiana, Villeneuve Pierre, Baréa Bruno, Yamamoto Carlos Itsuo, Côcco Lílian Cristina, Mitchell David Alexander, Krieger Nadia. 2010. Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11. Process Biochemistry (1991), 45 (8) : 1348-1354.

https://doi.org/10.1016/j.procbio.2010.05.004

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Quartile : Q1, Sujet : ENGINEERING, CHEMICAL / Quartile : Q2, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY / Quartile : Q3, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : We produced a lipase from Burkholderia cepacia in solid-state fermentation and used it to catalyze the synthesis of biodiesel in a fixed-bed reactor. In the solid-state fermentation step, a 50:50 (by mass) mixture of sugarcane bagasse and sunflower seed meal gave 234 units of pNPP-hydrolyzing activity per gram of dry solids at 96 h. This fermented solid was lyophilized and delipidated, packed into a column and used to catalyze the synthesis of biodiesel through the ethanolysis of soybean oil in a medium free of co-solvents, with the reaction mixture being continuously circulated through the column. The best conversion was 95% after 46 h, which was obtained at 50 ?C, with an alcohol:oil molar ratio of 3:1, alcohol addition in two steps and the addition of 1% of (m/m) water to the reaction medium. This system has potential to decrease the costs of enzyme-catalyzed transesterification reactions.

Mots-clés Agrovoc : Pseudomonas cepacia, Saccharum officinarum, Helianthus annuus, Glycine max, biocarburant, lipase

Classification Agris : P06 - Sources d'énergie renouvelable
Q60 - Traitement des produits agricoles non alimentaires

Champ stratégique Cirad : Axe 2 (2005-2013) - Biomasse énergie et sociétés du sud

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