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A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A

Song Yajun, Roumagnac Philippe, Weill François-Xavier, Wain John, Dolecek Christiane, Mazzoni Camila J., Holt Kathryn E., Achtman Mark. 2010. A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A. Journal of Antimicrobial Chemotherapy, 65 (8) : pp. 1631-1641.

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Quartile : Q1, Sujet : PHARMACOLOGY & PHARMACY / Quartile : Q1, Sujet : INFECTIOUS DISEASES / Quartile : Q1, Sujet : MICROBIOLOGY

Abstract : Objectives: Decreased susceptibility to fluoroquinolones has become a major problem for the successful therapy of human infections caused by Salmonella enterica, especially the life-threatening typhoid and paratyphoid fevers. Methods: By using Luminex xTAG beads, we developed a rapid, reliable and cost-effective multiplexed genotyping assay for simultaneously detecting 11 mutations in gyrA, gyrB and parE of S. enterica serovars Typhi and Paratyphi A that result in nalidixic acid resistance (NalR) and/or decreased susceptibility to fluoroquinolones. Results: This assay yielded unambiguous single nucleotide polymorphism calls on extracted DNA from 292 isolates of Salmonella Typhi (NalR¼223 and NalS¼69) and 106 isolates of Salmonella Paratyphi A (NalR¼24 and NalS¼82). All of the 247 NalR Salmonella Typhi and Salmonella Paratyphi A isolates were found to harbour at least one of the target mutations, with GyrA Phe-83 as the most common one (143/223 for Salmonella Typhi and 18/24 for Salmonella Paratyphi A). We also identified three GyrB mutations in eight NalS Salmonella Typhi isolates (six for GyrB Phe-464, one for GyrB Leu-465 and one for GyrB Asp-466), and mutations GyrB Phe-464 and GyrB Asp-466 seem to be related to the decreased ciprofloxacin susceptibility phenotype in Salmonella Typhi. This assay can also be used directly on boiled single colonies. Conclusions: The assay presented here would be useful for clinical and reference laboratories to rapidly screen quinolone-resistant isolates of Salmonella Typhi and Salmonella Paratyphi A, and decipher the underlying genetic changes for epidemiological purposes. (Résumé d'auteur)

Mots-clés Agrovoc : Salmonella, Genre humain, Santé publique, Typhoïde

Mots-clés géographiques Agrovoc : Asie, Afrique, Europe

Mots-clés complémentaires : Salmonella paratyphi a, Salmonella enterica, Salmonella typhi

Classification Agris : L73 - Animal diseases
000 - Other themes
U30 - Research methods

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

  • Song Yajun, UCC (IRL)
  • Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039
  • Weill François-Xavier, Institut Pasteur (FRA)
  • Wain John, HPA (GBR)
  • Dolecek Christiane, Oxford University (VNM)
  • Mazzoni Camila J., UCC (IRL)
  • Holt Kathryn E., Wellcome Trust Sanger Institute (GBR)
  • Achtman Mark, UCC (IRL)

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Source : Cirad - Agritrop (https://agritrop.cirad.fr/556480/)

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