Resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance

Brotman Yariv, Silberstein Leah, Kovalski Irina, Perin Christophe, Dogimont Catherine, Pitrat Michel, Klingler John, Thompson G.A., Perl-Treves Rafael. 2002. Resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance. Theoretical and Applied Genetics, 104 : 1055-1063.

Résumé : Genomic and cDNA fragments with homology to known disease resistance genes (RGH fragments) were cloned from Cucumis melo using degenerate-primer PCR. Fifteen homologues of the NBS-LRR gene family have been isolated. The NBS-LRR homologues show high divergence and, based on the partial NBS-fragment sequences, appear to include members of the two major subfamilies that have been described in dicot plants, one that possesses a TIR-protein element and one that lacks such a domain. Genomic organization of these sequences was explored by DNA gel-blot analysis, and conservation among other Cucurbitaceae was assessed. Two mapping populations that segregate for several disease and pest resistance loci were used to map the RGH probes onto the melon genetic map. Several NBS-LRR related sequences mapped to the vicinity of genetic loci that control resistance to papaya ringspot virus, Fusarium oxysporum race 1, F. oxysporum race 2 and to the insect pest Aphis gossypii. The utility of such markers for breeding resistant melon cultivars and for cloning the respective R-genes is discussed.

Mots-clés Agrovoc : Cucumis melo, résistance génétique, Aphis gossypii, Fusarium oxysporum, résistance aux organismes nuisibles, RFLP

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Brotman Yariv, Bar-Ilan University (ISR)
• Silberstein Leah, Bar-Ilan University (ISR)
• Kovalski Irina, Bar-Ilan University (ISR)
• Perin Christophe, INRA (FRA) ORCID: 0000-0002-2469-310X
• Dogimont Catherine, INRA (FRA)
• Pitrat Michel, INRA (FRA)
• Klingler John, University of Arizona (USA)
• Thompson G.A., University of Arkansas (USA)
• Perl-Treves Rafael, Bar-Ilan University (ISR)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/556643/)

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