Le Guen Vincent, Gay Céline, Xiong Tou Chen, Souza Livia Moura, Rodier-Goud Marguerite, Seguin Marc. 2011. Development and characterization of 296 new polymorphic microsatellite markers for rubber tree (Hevea brasiliensis). Plant Breeding, 130 (2) : 294-296.
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Quartile : Q2, Sujet : AGRONOMY / Quartile : Q2, Sujet : PLANT SCIENCES / Quartile : Q3, Sujet : BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Résumé : Enriched genomic libraries in GA and GT tandem repeat motifs were generated from rubber tree (Hevea brasiliensis). Design of appropriate primer pairs in the flanking regions enables to produce amplified fragments for 310 of these sequences. The test of these genomic microsatellites on a set of 10 rubber tree genotypes allows the characterization of 296 new polymorphic markers. Expected heterozygosity ranged from 0.10 to 0.93 and allele numbers from 2 to 11. Microsatellite loci with GA motifs were more abundant, longer and more polymorphic than GT motifs. Such a high variability suggests the utility of these markers for the establishment of integrated linkage maps, as well as the characterization of linkage disequilibrium in rubber tree populations.
Mots-clés Agrovoc : microsatellite, polymorphisme génétique, Hevea brasiliensis
Classification Agris : F30 - Génétique et amélioration des plantes
Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique
Auteurs et affiliations
- Le Guen Vincent, CIRAD-BIOS-UMR DAP (FRA)
- Gay Céline, INRA (FRA)
- Xiong Tou Chen, INRA (FRA)
- Souza Livia Moura, UNICAMP (BRA)
- Rodier-Goud Marguerite, CIRAD-BIOS-UMR DAP (FRA)
- Seguin Marc, CIRAD-BIOS-UMR DAP (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/559667/)
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