Development of novel microsatellite markers to identify the different invasive lineages in the Corbicula complex and to assess androgenesis

Pigneur Lise-Marie, Risterucci Ange-Marie, Dauchot Nicolas, Van Doninck K.. 2011. Development of novel microsatellite markers to identify the different invasive lineages in the Corbicula complex and to assess androgenesis. Molecular Ecology Resources, 11 (3) : 573-577.

Quartile : Q2, Sujet : ECOLOGY / Quartile : Q2, Sujet : EVOLUTIONARY BIOLOGY / Quartile : Q2, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Résumé : Reliable markers are needed to identify the lineages in the invasive clam genus Corbicula. Previous studies have demonstrated that mitochondrial (mt) DNA poorly resolves Corbicula phylogeny, owing to its androgenetic reproductive mode. Moreover, hybridization and mitochondrial ? nuclear mismatches occur. We developed the first eleven polymorphic markers to detect these phenomena and to investigate the nuclear identity of Corbicula populations. These microsatellite loci revealed three main lineages inWestern Europe. One locus allowed rapid discrimination of these three lineages on agarose gel, saving time and money. Moreover, the eleven markers were successfully cross-amplified in the invasive Corbicula lineages found in North America.

Mots-clés Agrovoc : Corbiculidae

Mots-clés géographiques Agrovoc : Europe occidentale

Classification Agris : L20 - Écologie animale
L10 - Génétique et amélioration des animaux
U30 - Méthodes de recherche

Champ stratégique Cirad : Hors axes (2005-2013)

Auteurs et affiliations

• Pigneur Lise-Marie, University of Namur (BEL)
• Risterucci Ange-Marie, CIRAD-BIOS-UMR DAP (FRA)
• Dauchot Nicolas, University of Namur (BEL)
• Van Doninck K., University of Namur (BEL)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/559668/)

Métriques

[ Page générée et mise en cache le 2024-10-23 ]