A reference linkage Map of C. clementina based on SNPs, SSRs and indels (P477)

Ollitrault Patrick, Terol Javier F., Chen Chunxian, Federici Claire T., Lotfy Samia, Hippolyte Isabelle, Ollitrault Frédérique, Bérard Aurélie, Chauveau Aurélie, Costantino Gilles, Kacar A.Yildiz, Mu Lisa, Cuenca José, Garcia Andres, Froelicher Yann, Aleza Pablo, Boland Anne, Billot Claire, Navarro Luis, Luro François, Roose Mikeal L., Gmitter Frederick G. Jr., Talon Manuel, Brunel Dominique. 2011. A reference linkage Map of C. clementina based on SNPs, SSRs and indels (P477). In : Abstracts of Plant and Animal Genomes XIXth Conference, San Diego, CA (USA), January 15-19, 2011. s.l. : s.n., Résumé Plant and Animal Genomes Conference. 19, San Diego, États-Unis, 15 Janvier 2011/19 Janvier 2011.

Résumé : A haploid C. clementina was chosen by the International Citrus Genomic Consortium (ICGC) to establish the reference whole genome sequence for Citrus. Development of a dense clementine linkage map was part of the objectives of this global collaborative project. Two inter-specific populations between C. clementina and C. maxima were used for this purpose. 156 hybrids of Nules clementine x Pink pummelo and 200 hybrids of Chandler pummelo x Nules clementine were genotyped with 1003 markers. 306 were SSRs markers (66 from genomic libraries, 207 from ESTs and 33 from clementine BAC end sequences -BES-), 34 were Indels markers mined from BES and 663 SNPs mined from Clementine BES or identified by candidate gene sequencing. 901 markers were successfully mapped in the 9 clementine linkage groups. Segregation distortion was observed for many loci of clementine when it was used as male parent while it followed Mendelian segregation for most markers when used as female parent. However marker order was mostly conserved between the male and female maps; thus, data of the two populations were joined to establish the reference clementine genetic map. The total clementine linkage map cover 1250 cM with linkage groups from 105 cM until 210 cM. This map is strongly anchored on a large diploid clementine BAC library resource. It is a powerful tool for Citrus genetics and supports the alignment of the haploid clementine whole genome sequence in the framework of the ICGC collaborative project.

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Ollitrault Patrick, CIRAD-BIOS-UMR AGAP (ESP) ORCID: 0000-0002-9456-5517
• Terol Javier F., IVIA (ESP)
• Chen Chunxian, University of Florida (USA)
• Federici Claire T., UCR (USA)
• Lotfy Samia, INRA [Maroc] (MAR)
• Hippolyte Isabelle, CIRAD-BIOS-UMR AGAP (FRA)
• Ollitrault Frédérique, IVIA (ESP)
• Bérard Aurélie, INRA (FRA)
• Chauveau Aurélie, INRA (FRA)
• Costantino Gilles, INRA (FRA)
• Kacar A.Yildiz, University of Cukurova (TUR)
• Mu Lisa, UCR (USA)
• Cuenca José, IVIA (ESP)
• Garcia Andres, IVIA (ESP)
• Froelicher Yann, CIRAD-BIOS-UMR AGAP (FRA)
• Aleza Pablo, IVIA (ESP)
• Boland Anne
• Billot Claire, CIRAD-BIOS-UMR AGAP (FRA)
• Navarro Luis, IVIA (ESP)
• Luro François, INRA (FRA)
• Roose Mikeal L., UCR (USA)
• Gmitter Frederick G. Jr., University de Florida (USA)
• Talon Manuel, IVIA (ESP)
• Brunel Dominique, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/559981/)

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