Zhe Li, Aihua Sun, Tiandai Huang, Quannan Zhou, Xuemei Dai, Huasun Huang, Weifu Lin, Lardet Ludovic, Montoro Pascal, Carron Marc-Philippe. 2010. Friable and embryogenic callus induction and plant regeneration from rubber tree clone Reyan88-13 Hevea brasiliensis. Chinese Journal of Tropical Crops, 31 (12) : 2166-2173.
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- Chinois
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Titre chinois : En chinois
Résumé : The inner integument from the young seeds of rubber tree clone Reyan88-13 was used to induce embryogenic callus. The different culture parameters such as the different types and concentrations of plant growth regulators and different calcium concentrations were studied in embryogenic callus induction media and subculture media. The proper culture parameters were determined. Friable and embryogenic callus were gradually obtained via continuous subculture and selection during 6 months. The suitable friable and embryogenic callus induction medium was: MH basal components, supplemented with 0.5 mg/L KT,2.0 mg/L 2,4-D, 11.0 mmol/L CaCl2, 80.0 g/L sucrose and 2.0 g/L Phytagel. Long-term subculture had been carried on for the friable and embryogenic calli. Histological sections proved that the calli proliferated by long -term subculture maintained the embryogenic characters. The friable and embryogenic calli of rubber tree clone Reyan88-13 that had been subcultured for more than 2 years were used for inducing somatic embryos. More than 16 000 somatic embryos were acquired, then 115 regeneration plantlets were obtained.
Mots-clés Agrovoc : Hevea brasiliensis, Embryogénèse somatique, régénération in vitro, clone, physiologie végétale
Mots-clés géographiques Agrovoc : Chine
Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement
F02 - Multiplication végétative des plantes
Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique
Auteurs et affiliations
- Zhe Li, CATAS (CHN)
- Aihua Sun, CATAS (CHN)
- Tiandai Huang, CATAS (CHN)
- Quannan Zhou, CATAS (CHN)
- Xuemei Dai, CATAS (CHN)
- Huasun Huang, CATAS (CHN)
- Weifu Lin, CATAS (CHN)
- Lardet Ludovic, CIRAD-BIOS-UMR DAP (FRA)
- Montoro Pascal, CIRAD-BIOS-UMR DAP (FRA)
- Carron Marc-Philippe, CIRAD-BIOS-UMR DAP (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/560039/)
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