Insertion sequence-and tandem repeat-base genotyping techniques for Xanthomonas citri pv. mangiferaeindicae

Pruvost Olivier, Vernière Christian, Vital Karine, Guérin Fabien, Jouen Emmanuel, Chiroleu Frédéric, Ah-You Nathalie, Gagnevin Lionel. 2011. Insertion sequence-and tandem repeat-base genotyping techniques for Xanthomonas citri pv. mangiferaeindicae. Phytopathology, 101 (7) : 887-893.

https://doi.org/10.1094/PHYTO-11-10-0304

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Quartile : Q1, Sujet : PLANT SCIENCES

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Molecular fingerprinting techniques that have the potential to identify or subtype bacteria at the strain level are needed for improving diagnosis and understanding of the epidemiology of pathogens such as Xanthomonas citri pv. mangiferaeindicae, which causes mango bacterial canker disease. We developed a ligation-mediated polymerase chain reaction targeting the IS1595 insertion sequence as a means to differentiate pv. mangiferaeindicae from the closely related pv. anacardii (responsible for cashew bacterial spot), which has the potential to infect mango but not to cause significant disease. This technique produced weakly polymorphic fingerprints composed of .70 amplified fragments per strain for a worldwide collection of X. citri pv. mangiferaeindicae but produced no or very weak amplification for pv. anacardii strains. Together, 12 tandem repeat markers were able to subtype X. citri pv. mangiferaeindicae at the strain level, distinguishing 231 haplotypes from a worldwide collection of 299 strains. Multilocus variable number of tandem repeats analysis (MLVA), IS1595-ligation-mediated polymerase chain reaction, and amplified fragment length polymorphism showed differences in discriminatory power and were congruent in describing the diversity of this strain collection, suggesting low levels of recombination. The potential of the MLVA scheme for molecular epidemiology studies of X. citri pv. mangiferaeindicae is discussed.

Mots-clés Agrovoc : Xanthomonas campestris citri, Mangifera indica

Mots-clés géographiques Agrovoc : La Réunion, France

Mots-clés complémentaires : Xanthomonas citri pv mangiferaeindicae

Classification Agris : H20 - Maladies des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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