Identification, characterization and mapping of EST-derived SSRs from cacao-Ceratocystis cacaofunesta interaction

Santos Rogério M.F., Gramacho Karina Peres, Clément Didier, Lemos L.S.L., Melo B.L.B., Juca F.F., Pires José Luis, Lopes Uilson Vanderlei, Micheli Fabienne. 2011. Identification, characterization and mapping of EST-derived SSRs from cacao-Ceratocystis cacaofunesta interaction. In : III Symposio Brasileiro de Genética Molecular de Plantas, 10 a 15 de abril de 2011, Ilhéus, BA. SBG. Ribeirão Preto : SBG, Résumé, 16. Simposio Brasileiro de Genética Molecular de Plantas. 3, Ilheus, Brésil, 10 Avril 2011/15 Avril 2011.

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Résumé : Ceratocystis cacaofunesta is an ascomycete responsible for the lethal wilt disease of cacao (Theobroma cacao L.). Marker-assisted selection combined with conventional breeding is one way in which the cacao resistance to Ceratocystis wilt can be improved. In this study, we screened a set of ESTs obtained from cacao elicited with C. cacaofunesta to identify ESTSSRs and test their efficacy for mapping. Among the 3,432 ESTs analyzed, 384 contained SSRs and 428 EST-SSRs were identified, mainly dinucleotides (78.5%), com 4 numbers of repeats (75.23%), and preferentially AG/CT motif (25.47%). GO function was assigned to the ESTs containing SSRs: 4.04% belonged to "defense response" category, with 20.69% of them to the sub-category "defense response to fungus". In relation to the ORF, the same amount of EST-SSRs was observed in 5'UTR as well as in the 3'UTR (about 30%). From the 428 EST-SSRs identified, 12 were polymorphic, revealing a total of 41 alleles. The number of alleles per locus ranged from 2 to 6, with an average of 3.41. Four EST-SSRs were mapped on the F2 Sca 6 x ICS 1 population segregating for Ceratocystis wilt, which were distributed on the linkage groups 2, 3, 4 and 8. These markers contributed to saturate the genetic map of the cacao mapping population from CEPEC/ CEPLAC and will be valuable for the research community to improve the cacao breeding program. (Texte intégral)

Mots-clés Agrovoc : Theobroma cacao, Ceratocystis fimbriata

Mots-clés géographiques Agrovoc : Brésil

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

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