Bambou Jean-Christophe, Gourdine Jean-Luc, Grondin Roxanne, Vachiery Nathalie, Renaudeau David. 2011. Effect of heat challenge on peripheral blood mononuclear cell viability : Comparison of a tropical and temperate pig breed. Tropical Animal Health and Production, 43 (8) : 1535-1541.
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Quartile : Q2, Sujet : VETERINARY SCIENCES
Résumé : We evaluated the effect of heat challenge on cell viability, concanavalin A-induced proliferation and heat shock protein (HSPs) mRNA expression in peripheral mononuclear blood cells (PBMC) isolated from Creole (CR) and Large White (LW) pigs. The PBMCs were cultured for 9 h at 37°C before being subjected to heat challenge: (1) at 42°C or 45°C for 2, 4, 6 and 9 h to monitor cell viability;(2) at 45°C for 2 and 9 h followed by stimulation for 24 h at 37°C with concanavalin A to evaluate mitogen-induced proliferation; and (3) at 45°C for 3, 6 and 9 h to measure induction of HSP70.2 and HSP90 mRNA. Cell viability was affected by breed and temperature (P<0.01), and the viability decrease caused by heat challenge was greater for LW than CR pigs. For mitogenstimulated PBMCs, incubation at 45°C reduced lymphoblastogenesis equally in both breeds (P<0.01). Although heat challenge for 3 and 6 h at 45°C induced expression of HSP70.2 and HSP90 mRNA, no breed difference was observed. In conclusion, differences in heat resistance between these two breeds at the whole organism level are reflected at the cellular level. Neither HSP70.2 nor HSP90 mRNA expression levels explain this effect.
Mots-clés Agrovoc : porcin, tolérance à la chaleur, climat tropical, viabilité, cellule sanguine, race indigène, race (animal)
Mots-clés géographiques Agrovoc : Guadeloupe, France
Mots-clés complémentaires : Porc Créole
Classification Agris : L52 - Physiologie animale - Croissance et développement
L50 - Physiologie et biochimie animales
Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique
Auteurs et affiliations
- Bambou Jean-Christophe, INRA (GLP)
- Gourdine Jean-Luc, INRA (GLP)
- Grondin Roxanne, INRA (GLP)
- Vachiery Nathalie, CIRAD-BIOS-UMR CMAEE (GLP)
- Renaudeau David, INRA (GLP)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/562804/)
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