Nineteen polymorphic microsatellite markers from two african Vanilla species: across-species transferability and diversity in a wild population of V. humblotii from Mayotte

Gigant Rodolphe, Brugel Anaïs, De Bruyn Alexandre, Risterucci Ange-Marie, Guiot Valérie, Viscardi Guillaume, Humeau Laurence, Grisoni Michel, Besse Pascale. 2012. Nineteen polymorphic microsatellite markers from two african Vanilla species: across-species transferability and diversity in a wild population of V. humblotii from Mayotte. Conservation Genetics Resources, 4 (1) : 121-125.

https://doi.org/10.1007/s12686-011-9489-1

Article de revue ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
document_563439.pdf
Télécharger (180kB)

Quartile : Q3, Sujet : BIODIVERSITY CONSERVATION / Quartile : Q4, Sujet : GENETICS & HEREDITY

Résumé : There is a serious lack of information on the genetic diversity and population dynamics of the 110 tropical Vanilla species, although these are keys elements to adjust conservation strategies. Nineteen polymorphic microsatellite markers were developed from two African leafless Vanilla species V. roscheri and V. humblotii to use in population genetic studies. A transferability analysis of these markers on seven Vanilla species from various geographical origins was conducted. Nine microsatellites were polymorphic in a population of 22 individuals of V. humblotii from Mayotte (Comoros Archipelago). These markers had two to eight alleles per locus and observed heterozygosity ranged from 0.23 to 0.64. Amplification parameters were calibrated to facilitate multiplexing and rapid multi-loci genotyping.

Mots-clés Agrovoc : Vanilla, microsatellite, dynamique des populations, conservation des ressources, variation génétique

Mots-clés géographiques Agrovoc : La Réunion, Comores, France

Mots-clés complémentaires : Vanilla humblotii, Vanilla roscheri

Classification Agris : F30 - Génétique et amélioration des plantes
F70 - Taxonomie végétale et phytogéographie

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations