Gek-Lan Chi, Hedy K.L. Goh, Kuwan-Yee Hoo, Legavre Thierry.
1998. GUS gene expression in Anthurium andreanum, Oncidium grower ramsey and Brassolaeliocattleya orange glory empress after particle bombardment.
In : Proceedings of the First International symposium on biotechnology of tropical and subtropical species, Brisbane, Queensland, Australia, September 29-October 3, 1997. Part 2. Drew R.A. (ed.). ISHS
Résumé : Different plasmids (pUGCI - maize ubiquitine promoter; pEF-1alpha- Arabidopsis thaliana promoter; and p70GUS - CaMV promoter) carrying the various promoters driving expression of the bacterial gene encoding for beta-glucuronidase were coated onto gold microcarriers and used for bombardment with the Biolistic PSD-1000/He system operated at 900 psi, 1100 psi, 1300 psi or 1550 psi. The target tissues used were callus and whole leaves from Anthurium andreanum and protocorms from Oncidium and Brassolaeliocattleya which are highly regenerative. Transient GUS expression was monitored 24 h or 48 h post-bombardment. The highest transient expression of GUS in A. andreanum callus tissues were obtained using pUGCI. This indicates that the promoter from maize, a monocot, is more efficient in expressing the GUS gene in Anthurium than dicot promoters. In Oncidium, both pUGCI and pEF-1alpha gave high GUS activity. For Brassolaeliocattleya protocorms, pEF-1alpha and pUGCI gave similar GUS activity per protocorm.
Mots-clés Agrovoc : Anthurium andraeanum, Oncidium, Orchidaceae, génétique moléculaire, expression des gènes
Mots-clés complémentaires : GUS
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Gek-Lan Chi, Ngee Ann Polytechnic (SGP)
- Hedy K.L. Goh, Ngee Ann Polytechnic (SGP)
- Kuwan-Yee Hoo, Ngee Ann Polytechnic (SGP)
- Legavre Thierry, CIRAD-GERDAT-BIOTROP/CIV (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/563776/)
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