Isolation and characterization of twelve polymorphic microsatellite loci for the cocoa mirid bug Sahlbergella Singularis

Babin Régis, Fenouillet Catherine, Legavre Thierry, Blondin Laurence, Calatayud Caroline, Risterucci Ange-Marie, Chapuis Marie-Pierre. 2012. Isolation and characterization of twelve polymorphic microsatellite loci for the cocoa mirid bug Sahlbergella Singularis. International Journal of Molecular Sciences, 13 (4) : 4412-4417.

https://doi.org/10.3390/ijms13044412

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Quartile : Q2, Sujet : CHEMISTRY, MULTIDISCIPLINARY

Résumé : Mirids are the primary pests affecting cocoa production in Africa, but no genetic studies have been conducted on these insects. Here we report the isolation and characterization of 12 polymorphic microsatellite loci for Sahlbergella singularis. A microsatellite-enriched genomic DNA library was developed and screened to identify marker loci. Twelve polymorphic loci were identified by screening 28 individuals collected from one presumed population in cocoa plantations in Southern Cameroon. The number of alleles ranged from 5 to 25, whereas the observed and the expected heterozygosities ranged from 0.179 to 0.786 and from 0.671 to 0.946, respectively. Tests showed significant deviations from HW equilibrium for four loci, but no linkage disequilibrium was detected at any of the loci. No cross-species amplification was observed in two other mirid pests in Africa.

Mots-clés Agrovoc : Sahlbergella, Miridae, Theobroma cacao, Sorghum, microsatellite, marqueur génétique, polymorphisme génétique

Mots-clés géographiques Agrovoc : Cameroun, Afrique

Mots-clés complémentaires : Sahlbergella singularis, Eurystylus oldi, Distantiella theobromae

Classification Agris : H10 - Ravageurs des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

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