Molecular homology and docking of a natural cysteine-proteinase and cystatin from cacao : [Abstract R9164]

Cardoso Thyago Hermylly Santana, Andrade Bruno Silva, Santiago A.S., Dias Cristiano Villela, Koop Daniela Martins, Micheli Fabienne, Goes-Neto Aristoteles, Cascardo Julio Cezar M., Alvim Fatima Cerqueira, Pirovani Carlos Priminho. 2011. Molecular homology and docking of a natural cysteine-proteinase and cystatin from cacao : [Abstract R9164]. In : XL Annual Meeting of Brazilian Biochemistry and Molecular Biology Society, Foz do Iguacu, Brésil, April 30th to May 3rd, 2011. s.l. : s.n., Résumé, 1 p. Annual Meeting of Brazilian Biochemistry and Molecular Biology Society. 40, Foz do Iguaca, Brésil, 30 Avril 2011/3 Mai 2011.

Résumé : Cysteine-proteinases and cystatins are proteins related with defense mechanisms against pathogen attack, and involved in the endogenous regulation of programmed cell death. Genes of both proteins were encountered in T. cacao - M. perniciosa interaction cDNA library. Bioinformatics analysis revealed that the cysteine protease contains an amino terminus signal peptide with probable cleavage site after the 19th aa followed by a conserved auto-inhibitory domain of 56 aa and a catalytic domain of 218 aa, characteristic of the C1 peptidase family. The cystatin (205 aa) contains a conserved phytocystatin motif and a site inhibitory of proteinase. Modeling homology of both proteins using Modeller9 v.8 software showed that the cysteine-proteinase needs to lose its inhibitory domain to become active and leave the catalytic cleft exposed to substrate. Molecular docking using ClusPro 2.0 software revealed the in silico interaction between the enzyme and inhibitor from cacao. This result was confirmed by experimental capture of the proteinase using the cystatin followed by the identification of the captured protein by mass spectrometry. Thus, we evidenced by in silico and in vitro analysis that these two proteins interact, balancing their performance at cellular level in response to different stimuli such M. perniciosa infection.

Mots-clés Agrovoc : Theobroma cacao, Moniliophthora, cystéine, inhibiteur de protéinases

Mots-clés géographiques Agrovoc : Bahia

Mots-clés complémentaires : Moniliophthora perniciosa

Classification Agris : F60 - Physiologie et biochimie végétale
H20 - Maladies des plantes

Auteurs et affiliations

• Cardoso Thyago Hermylly Santana, UESC (BRA)
• Andrade Bruno Silva, UESB (BRA)
• Santiago A.S., UESC (BRA)
• Dias Cristiano Villela, UESC (BRA)
• Koop Daniela Martins, UESC (BRA)
• Micheli Fabienne, CIRAD-BIOS-UMR AGAP (BRA)
• Goes-Neto Aristoteles
• Cascardo Julio Cezar M., UESC (BRA)
• Alvim Fatima Cerqueira, UESC (BRA)
• Pirovani Carlos Priminho, UESC (BRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/564007/)

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