Transgenic plants over-expressing HbCuZnSOD cytosolic isoform are more tolerant to a water deficit

Leclercq Julie, Martin Florence, Sanier Christine, Clément-Vidal Anne, Fabre Denis, Oliver Gérald, Lardet Ludovic, Ayar Ayan, Peyramard Mathieu, Montoro Pascal. 2011. Transgenic plants over-expressing HbCuZnSOD cytosolic isoform are more tolerant to a water deficit. In : IRRDB International Rubber Conference, 14-17 December 2011, Chaing Mai, Thailand. IRRDB. s.l. : s.n., 3 p. IRRDB International Rubber Conference, Chiang Mai, Thaïlande, 14 Décembre 2011/17 Décembre 2011.

Résumé : Reactive oxygen species (ROS) scavenging systems are involved in various biotic and abiotic stresses. Genetic engineering of Hevea was attempted to strengthen plant defences against ROS accumulation. Hevea transgenic plant lines over-expressing cytosolic HbCuZnSOD gene driven by a 35S CAMV promoter were successfully established and regenerated. The overexpression affected somatic embryogenesis and in plant development. The water deficit tolerance of two fast-growing HbCuZnSOD over-expressing lines (TS5T3Af and TS4T8An) was evaluated. Plants from line TS4T8An displayed lower stomatal conductance, a higher proline content and an activation of all ROS-scavenging enzymes suggesting a better protection against ROS.

Mots-clés Agrovoc : plante transgénique, Hevea brasiliensis, résistance à la sécheresse

Classification Agris : F30 - Génétique et amélioration des plantes
F62 - Physiologie végétale - Croissance et développement
H50 - Troubles divers des plantes

Auteurs et affiliations

• Leclercq Julie, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0001-8110-3762
• Martin Florence, CIRAD-BIOS-UMR AGAP (FRA)
• Sanier Christine, CIRAD-BIOS-UMR AGAP (FRA)
• Clément-Vidal Anne, CIRAD-BIOS-UMR AGAP (FRA)
• Fabre Denis, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-6222-2587
• Oliver Gérald, CIRAD-BIOS-UMR AGAP (FRA)
• Lardet Ludovic, CIRAD-BIOS-UMR AGAP (FRA)
• Ayar Ayan
• Peyramard Mathieu
• Montoro Pascal, CIRAD-BIOS-UMR AGAP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/564414/)

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