The direct protein-protein interaction results in the arms race co-evolution between Magnaporthe oryzae AVR-Pik and rice Pik

Kanzaki Hiroyuki, Yoshida Kentaro, Saitoh Hiromasa, Hirabuchi Akiko, Alaux Ludovic, Fournier Elisabeth, Tharreau Didier, Terauchi Ryohei. 2012. The direct protein-protein interaction results in the arms race co-evolution between Magnaporthe oryzae AVR-Pik and rice Pik. In : IS-MPMI 2012 XV International Congress, program and abstracts, July 29 (Sun.) - August 2 (Thu), 2012. Kyoto, Japan. IS-MPMI. Saint-Paul : IS-MPMI, Résumé, 200. International Congress on Molecular Plant-Microbe Interactions. 15, Kyoto, Japon, 29 Juillet 2012/2 Août 2012.

Résumé : Between pathogen and host, antagonistic interactions impose strong reciprocal selection on each organism, leading to the development of arms race evolutionary dynamics. However, studies on specific recognition and co-evolution between resistance (R-) gene and avirulence (AVR-) gene are still limited. Here we show that AVRPik of Magnaporthe oryzae, the rice blast pathogen, and cognate rice R-gene Pik exhibit high levels of DNA polymorphisms causing amino acid changes. We found a tight recognition specificity of AVRPik alleles by different Pik alleles. Pik is composed of two kinds of CC-NBS-LRR, Pik1 and Pik2. We found that AVR-Pik physically interacts with the N-terminal coiled-coil domain of Pik1 in yeast 2-hybrid assay as well as in in-planta co-immunoprecipitation assay. Furthermore, this binding specificity corresponds to the recognition specificity between AVR-Pik and Pik alleles. These data suggest that the direct protein-protein interaction results in the arms race co-evolution between AVR-Pik and Pik. (Texte intégral)

Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Kanzaki Hiroyuki, IBRC (JPN)
• Yoshida Kentaro, IBRC (JPN)
• Saitoh Hiromasa, IBRC (JPN)
• Hirabuchi Akiko, IBRC (JPN)
• Alaux Ludovic
• Fournier Elisabeth, INRA (FRA)
• Tharreau Didier, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-3961-6120
• Terauchi Ryohei, IBRC (JPN)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/565196/)

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